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目的探讨子宫颈癌术中髂内动脉灌注顺铂对宫颈癌的近期疗效及其作用机制。方法用免疫组织化学法及TdT介导的dUTP缺口末端标记技术,检测25例术中行髂内动脉灌注化疗的子宫颈癌患者(灌注组)肿瘤组织中癌细胞的凋亡及增殖情况,并选择同期手术治疗的宫颈癌患者25例(对照组)作为对照。结果灌注组肿瘤细胞的细胞增殖指数(PI)为(46.34±18.76)%,略低于对照组的(58.48±16.54)%(P>0.05),灌注组中肿瘤细胞的凋亡指数(AI)为(17.67±10.32)%,明显高于对照组的(6.92±5.76)%(P<0.05)。结论子宫颈癌术中通过髂内动脉灌注化疗,可以诱导子宫颈癌细胞发生凋亡,并可能降低其增殖活力。
Objective To investigate the short-term effect and mechanism of intra-arteria iliac artery perfusion of cisplatin on cervical cancer during cervical intraepithelial neoplasia. Methods Immunohistochemistry and TdT-mediated dUTP nick end labeling were used to detect the apoptosis and proliferation of cancer cells in 25 cases of intra-iliac arterial infusion chemotherapy in patients with cervical cancer (perfusion group) The same period surgical treatment of cervical cancer in 25 patients (control group) as a control. Results The proliferation index (PI) of tumor cells in perfusion group was (46.34 ± 18.76)%, slightly lower than that in control group (58.48 ± 16.54)% (P> 0.05). The apoptosis index (17.67 ± 10.32)%, which was significantly higher than that of the control group (6.92 ± 5.76)% (P <0.05). Conclusion Intra-arteria iliac artery infusion of chemotherapy can induce apoptosis of cervical cancer cells and may reduce their proliferative activity.