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目的研究粗壮女贞对双氧水损伤人血管内皮细胞的保护作用。方法不同浓度H_2O_2处理HUVEC细胞构建氧化损伤模型,将细胞分为正常组、H_2O_2损伤组和不同浓度粗壮女贞组。采用CCK-8法测各组细胞活性,并测定各组细胞LDH、SOD、CAT、MDA和GSH-Px的活性。结果 500μmol/L H_2O_2为构建氧化损伤HUVEC细胞模型的最适浓度。粗壮女贞在测试浓度下对细胞不具有毒性作用。与H_2O_2损伤组相比,粗壮女贞干预组细胞存活率明显增加(P<0.05);粗壮女贞干预组LDH、MDA活性明显降低(P<0.05);SOD、CAT、GSH-Px活性明显增加(P<0.05)。结论粗壮女贞对氧化损伤的HUVEC细胞具有保护效应。
Objective To study the protective effect of Prunus nucifera on human vascular endothelial cells injured by hydrogen peroxide. Methods HUVEC cells were treated with different concentrations of H 2 O 2 to establish an oxidative injury model. The cells were divided into normal group, H 2 O 2 -induced group and prunella group with different concentrations. The activity of LDH, SOD, CAT, MDA and GSH-Px in each group were measured by CCK-8 method. Results 500μmol / L H 2 O 2 was the optimal concentration for the construction of oxidatively injured HUVECs. Prunella vulgaris does not have toxic effects on the cells at test concentrations. Compared with H 2 O 2 -induced group, the cell viability in the treatment group was significantly increased (P <0.05); the activity of LDH and MDA in the treatment group was significantly decreased (P <0.05); the activities of SOD, CAT and GSH-Px were significantly increased (P <0.05). Conclusion Stout virginia has a protective effect on oxidatively injured HUVECs.