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取灌喂克癌7851(160 mg/kg)20d的荷HepA肝癌小鼠的腹水癌细胞进行细胞生物学特性的研究,把经过一系列处理和荧光染色的癌细胞在FACS 420型流式细胞光度计上进行分析。结果表明:①克癌7851可使癌细胞的DNA含量明显下降(从1511.7±1.2降至133.5±4.4 AU或从157.0±1.7降至146.0±3.0 AU);②DAN指数(DI值)和增殖指数(PI值)亦明显下降(p<0.05和p<0.01);③可使癌细胞的G1期细胞数明显增加,从43.2±4.5％增加到78.3±5.5％,而S期和G 2M期的细胞数明显减少,而且DNA组方图的多倍体峰变得低平。提示克癌7851可以抑制癌细胞的DNA合成。
The ascites cancer cells of hepatocarcinoma-bearing mice bearing Hepatocarcinoma 7851 (160 mg/kg) for 20 days were studied for cell biological characteristics, and the cells treated with a series of treatments and fluorescent staining were subjected to FACS 420 flow cytometry. Calculated for analysis. The results showed that: 1 g of cancer 7851 can significantly reduce the DNA content of cancer cells (from 1511.7 ± 1.2 to 133.5 ± 4.4 AU or from 157.0 ± 1.7 to 146.0 ± 3.0 AU); 2 DAN index (DI value) and proliferation index ( The PI value also decreased significantly (p<0.05 and p<0.01); 3 the number of G1 phase cells in cancer cells increased significantly from 43.2±4.5% to 78.3±5.5%, while the S phase and G2M phase cells The number is significantly reduced, and the polyploid peak of the DNA histogram becomes flat. Tip Kewei 7851 can inhibit the DNA synthesis of cancer cells.