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目的：研究白细胞（WBC）参与缺血再准流肾损伤的作用高峰时间。方法：将24只家兔随机分为对照、缺血再灌流2小时（IR－2小时）和缺血再灌流24小时（IR－24小时）三组，分别观察血中白细胞（WhC）和过氧化脂质（LPO）含量、左肾系数（LRC）和肾超微结构的改变。结呆：IR－2小时组同对照组比较，WBC和LPO含量明显升高，LRC明显增大（P＜0．05），且WBC与LPO、LRC之间均呈显著正相关（P＜0．05）。IR-24小时组在24小时时，WBC与LPO之间仍呈显著正相关（P＜0．05），但WBC与LRC之间无相关关系，同IR-2小时时比较，WBC和LPO含量明显下降（P＜0．05）；同对照组比较，LRC明显增大（P＜0．05）。IR-2小时和IR-24小时组肾组织超微结构改变一致，肾小球和肾小管均发生明显的变性、坏死。结论：白细胞参与肾损伤，其作用的高峰期在再灌流2小时左右。
Objective: To study the role of leukocyte (WBC) in the peak time of ischemic re-quasi-renal injury. Methods: Twenty-four rabbits were randomly divided into control group, ischemia-reperfusion 2 hours (IR-2 hours) and ischemia-reperfusion 24 hours (IR 24 hours) LPO content, left renal coefficient (LRC) and renal ultrastructure changes. Results: Compared with the control group, the levels of WBC and LPO in the IR-2 hour group increased significantly, LRC increased significantly (P <0.05), and there was a significant positive correlation between WBC and LPO and LRC .05). At 24 hours, there was a significant positive correlation between WBC and LPO (P <0.05), but no correlation was found between WBC and LRC. Compared with IR-2 hours, WBC and LPO contents (P <0.05). Compared with the control group, LRC increased significantly (P <0.05). IR-2 hours and IR-24 hours of renal tissue ultrastructural changes consistent with glomerular and renal tubules were significantly degeneration and necrosis. CONCLUSION: Leukocytes are involved in renal injury and their peak period is about 2 hours after reperfusion.