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目的:研究白细胞(WBC)参与缺血再准流肾损伤的作用高峰时间。方法:将24只家兔随机分为对照、缺血再灌流2小时(IR-2小时)和缺血再灌流24小时(IR-24小时)三组,分别观察血中白细胞(WhC)和过氧化脂质(LPO)含量、左肾系数(LRC)和肾超微结构的改变。结呆:IR-2小时组同对照组比较,WBC和LPO含量明显升高,LRC明显增大(P<0.05),且WBC与LPO、LRC之间均呈显著正相关(P<0.05)。IR-24小时组在24小时时,WBC与LPO之间仍呈显著正相关(P<0.05),但WBC与LRC之间无相关关系,同IR-2小时时比较,WBC和LPO含量明显下降(P<0.05);同对照组比较,LRC明显增大(P<0.05)。IR-2小时和IR-24小时组肾组织超微结构改变一致,肾小球和肾小管均发生明显的变性、坏死。结论:白细胞参与肾损伤,其作用的高峰期在再灌流2小时左右。
Objective: To study the role of leukocyte (WBC) in the peak time of ischemic re-quasi-renal injury. Methods: Twenty-four rabbits were randomly divided into control group, ischemia-reperfusion 2 hours (IR-2 hours) and ischemia-reperfusion 24 hours (IR 24 hours) LPO content, left renal coefficient (LRC) and renal ultrastructure changes. Results: Compared with the control group, the levels of WBC and LPO in the IR-2 hour group increased significantly, LRC increased significantly (P <0.05), and there was a significant positive correlation between WBC and LPO and LRC .05). At 24 hours, there was a significant positive correlation between WBC and LPO (P <0.05), but no correlation was found between WBC and LRC. Compared with IR-2 hours, WBC and LPO contents (P <0.05). Compared with the control group, LRC increased significantly (P <0.05). IR-2 hours and IR-24 hours of renal tissue ultrastructural changes consistent with glomerular and renal tubules were significantly degeneration and necrosis. CONCLUSION: Leukocytes are involved in renal injury and their peak period is about 2 hours after reperfusion.