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目的探讨新型抑癌基因p33ING1真核表达质粒在胃癌细胞株中的表达及对胃癌细胞株的生长抑制、凋亡的作用,探索新型肿瘤治疗措施与方法。方法构建PCDNA3/p33ING1真核表达质粒,将p33ING1和wt-p53单、共染至人胃癌细胞,研究p33ING1与wt-p53间协同功能及对胃癌细胞产生的作用。结果重组PCDNA3/p33ING1真核表达质粒构建成功,经p33ING1和wt-p53单、共染的人胃癌细胞株SSCG-7901生长速度减慢,融合时间变长,周期S期变短,G0/G1期延长,凋亡增加。结论p33ING1除本身具有抑癌功能及生物活性外,同时参与p53基因并与其一起调控胃癌细胞的生长,诱导细胞的凋亡,使细胞周期阻滞,二者为相互依赖协同作用。
Objective To investigate the expression of p33ING1 eukaryotic expression plasmid in gastric cancer cell lines and its effect on the growth inhibition and apoptosis of gastric cancer cell lines and to explore novel tumor treatment measures and methods. Methods The eukaryotic expression vector pcDNA3 / p33ING1 was constructed and co-transfected with p33ING1 and wt-p53 into human gastric cancer cells. The synergistic effect between p33ING1 and wt-p53 and the effect on gastric cancer cells were studied. Results The recombinant eukaryotic expression vector pcDNA3 / p33ING1 was successfully constructed. The single cell co-transfected p33ING1 and wt-p53 human gastric cancer cell line SSCG-7901 slowed down, the fusion time became longer, the period of S phase became shorter, and the G0 / G1 phase Prolonged, increased apoptosis. Conclusion p33ING1 participates in the p53 gene in addition to its own tumor suppressor and biological activity, together with its regulation of the growth of gastric cancer cells, induces cell apoptosis and cell cycle arrest, both of which are interdependent synergistic effects.