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有许多研究显示RNaseH参与了反义寡聚脱氧核苷酸(ODN)抑制靶基因翻译的过程,它能降解互补的RNA/DNA双链中的RNA。非修饰的15聚体ODN1与免α球蛋白mRNA(α-mRNA)完全互补,与β球蛋白mRNA(β-mRNA)至少有60％的部分互补。由ODN1经过两端不同程度的甲基化分别形成ODN2和ODN3两种三明治式的寡聚物(MPSO),它们中间非修饰的脱氧核苷酸分别为5个和7个。作者将α、β-mRNA分别与ODN1、ODN2和ODN3加到富含RNaseH的体外翻译系统麦胚提取物中培养,然后PAGE电泳和放射性自显影。结果,0.3～0.5μmol/L ODNI
Numerous studies have shown that RNaseH is involved in the antisense oligodeoxynucleotides (ODNs) that inhibit target gene translation and degrade RNA in complementary RNA / DNA duplexes. Unmodified 15-mer ODN1 is perfectly complementary to α-globin mRNA (α-mRNA) and partially complementary to at least 60% of β-globin mRNA (β-mRNA). ODN1 was methylated to form two kinds of sandwich oligomers (MPSO) of ODN2 and ODN3 respectively. There were 5 and 7 non-modified deoxynucleotides among them. The authors added α, β-mRNA to ODN1, ODN2 and ODN3, respectively, to wheat germ extracts enriched with RNaseH in vitro translation system, followed by PAGE and autoradiography. As a result, 0.3 to 0.5 μmol / L ODNI