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用珍汕97B/密阳46构建RIL群体及其遗传图谱,经海南陵水和浙江杭州两地遗传试验,以精米粉RVA谱5个参数特征值PKV、HPV、CPV、BDV和SBV作为研究稻米淀粉黏滞性的指标,运用检测QTL主效应、上位性效应和G×E互作效应的遗传分析方法,进行QTL联合分析。结果表明,(1)在检测到涉及5个性状的9个主效应QTL中,除PKV位于第5染色体qPKV5外,其余8个QTL均位于第6染色体上;(2)5个性状均检测到位于第6染色体RM197-RZ516区间的主效应QTL,很可能它们为同一基因,该基因还与Wx基因处于相同区域;(3)检测到与PKV、HPV、CPV、BDV等4个性状有关的QTL主效基因均表现有G×E互作,且方向一致,在海南试验中有增效作用;(4)还检测到涉及5个性状的10对上位性互作效应,但均未发现有显著的上位性×环境互作效应。
The RIL population and its genetic map were constructed by Zhenshan 97B / Miryang 46, and the genetic test was conducted in Lingshui, Hainan Province and Hangzhou, Zhejiang Province. The five parameters of RVA profile of polished rice flour (PKV, HPV, CPV, BDV and SBV) Starch viscosity indicators, the use of detection of the main effects of QTL, Epistasis and G × E interaction effects of genetic analysis methods for QTL joint analysis. The results showed that: (1) Among the nine major QTLs detected in five traits, all the eight QTLs were located on chromosome 6 with the exception of PKV on qPKV5. (2) Five traits were detected The main QTLs located in the region of RM197-RZ516 on chromosome 6 are likely to be the same gene, which is also located in the same region as the Wx gene. (3) QTLs related to 4 traits such as PKV, HPV, CPV and BDV were detected The major genes all showed G × E interaction with the same orientation and synergism in Hainan experiment. (4) Ten pairs of epistatic effects involving five traits were also detected, but none of them were found significant Epistasis × environment interaction effect.