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为研究长期滴用血管收缩滴鼻剂后呼吸区鼻粘膜组织病理学和组织化学的变化,用0.05%盐酸萘唑啉(鼻眼净)2滴,滴入豚鼠鼻腔,每日三次,分别在2、4、6、8、12和16周时处死,手术显微镜下剖出呼吸区鼻粘膜,一部分固定在Bouin氏液,制成石蜡切片,用(1)苏木精和伊红染色,作组织病理学研究;(2)对氨基水杨酸-阿辛蓝染色,作粘多糖着色。另一部分制成冰冻切片,进行①琥珀酸脱氢酶、②α-酯酶、
In order to study the histopathological and histochemical changes of nasal mucosa in nasal mucosa of nasal mucosa after nasal drops with long-term vasoconstriction, two drops of naphazoline hydrochloride (net of nasal eye) were applied to nasal cavity of guinea pigs three times daily, The animals were sacrificed at days 2, 4, 6, 8, 12 and 16, and the nasal mucosa of the respiratory area was dissected under a surgical microscope. Part of the nasal mucosa was fixed in Bouin’s solution and paraffin sections were prepared and stained with (1) hematoxylin and eosin Histopathological studies; (2) Aminosalicylic acid - Alcian blue staining for mucopolysaccharide staining. The other part made of frozen sections, ① succinate dehydrogenase, ② α-esterase,