利用生物信息学的方法,对10个苹果MdCBLs家族蛋白的功能域进行预测,并与拟南芥AtCBLs家族的10个蛋白进行了系统进化分析,同时对苹果MdCBLs基因进行了系统的命名。利用qRT-PCR,检测了苹果MdCBLs基因在不同组织的表达及对非生物胁迫(包括盐、低温、ABA、干旱)的响应,结果表明,MdCBLs在苹果不同组织及非生物胁迫中起重要作用。另外,通过遗传转化苹果愈伤组织鉴定了MdCBL1在盐胁迫中的功能,MdCBL1过量表达明显提高了转基因愈伤组织的盐胁迫抗性。 Bioinformatics methods were used to predict the functional domains of 10 apple MdCBLs family proteins. Phylogenetic analysis was performed on 10 proteins of the AtCBLs family in Arabidopsis. Meanwhile, the apple MdCBLs genes were systematically named. QRT-PCR was used to detect the expression of MdCBLs in different tissues and the responses to abiotic stress (including salt, hypothermia, ABA, drought). The results showed that MdCBLs played an important role in different tissues and abiotic stress in apples. In addition, the function of MdCBL1 in salt stress was identified by genetic transformation of apple callus. Overexpression of MdCBL1 significantly enhanced the salt stress resistance of transgenic callus.