This paper aims to discuss the application of eye surgery preparation room (ESPR),also called eye preoperative holding area, in nursing safety assessment and the surgery preparationbefore ophthalmologic operation. The establishment and application of ESPR optimizes theprocess of ophthalmologic surgery as well as decreases the surgery-waiting time of patients andtover period of surgical operations, which improves the utilization efficiency of surgery operatingroom. In addition, the application of ESPR also guarantees the safety of patients during surgicaloperations and enhances the doctors satisfaction of cooperation and coordination in surgeryoperating room.