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目的：确定云南省恶性疟原虫裂殖子表面蛋白１（ Ｍ Ｓ Ｐ １）基因分型和探讨 Ｍ Ｓ Ｐ １ 基因多态性的遗传及地理特征。方法：采用巢式 Ｐ Ｃ Ｒ法和引物标记周期反应测定法，对云南疫区恶性疟原虫群体 Ｍ Ｓ Ｐ１ 基因分型，并对代表株进行基因序列分析。结果：３０ 例云南恶性疟患者，检出３８ 个基因型虫株，其中 Ｍ Ａ Ｄ２０ 型是优势虫株， Ｋ１ 次之， Ｒ Ｏ３３ 最少，并存在不同基因株混合感染现象。扩增片段序列分析表明，云南疫区的 Ｍ Ａ Ｄ２０ 型、 Ｋ１ 型和 Ｒ Ｏ３３型均分别与国际上典型的 Ｍ Ｓ Ｐ１、 Ｍ Ａ Ｄ ２０、 Ｋ１ 和 Ｒ Ｏ ３３ 等位基因代表株具有高度的同源性。结论：以 Ｍ Ｓ Ｐ１ 为基因标记物的基因分型法，有助于掌握流行区疟原虫种群的基因特点、分布及流行特征。
Objective: To determine the genotypes of MSP1 gene of Plasmodium falciparum in Yunnan Province and to explore the genetic and geographical features of MSP1 gene polymorphism. Methods: The nested P C R method and primer-labeled periodic reaction assay were used to genotype M S P1 of P. falciparum population in Yunnan Province. The sequence analysis of the representative strains was carried out. Results: Thirty-eight genotypes were detected in 30 cases of P. falciparum in Yunnan. Among them, M A D20 was the dominant strain, followed by K1, with the lowest R O33 and mixed infection with different strains. Sequence analysis of the amplified fragments showed that the M A D20, K1 and R O33 genotypes in the Yunnan endemic areas were respectively highly homologous to the representative strains of the M S P1, M A D 20, K1 and R O 33 alleles in the world. Homology. Conclusion: The genotyping method with M S P1 as a gene marker can help to understand the characteristics, distribution and epidemic characteristics of the malaria parasite population in endemic areas.