The subtropical mixed broad-leaved and coniferous forest, a typical successional monsoon forest, is one of the major forests in the subtropics of China. Therefore, it is very important to estimate the fluxes of the greenhouse gases from the forest soil in order to evaluate the impact of subtropical forests on the greenhouse gas emissions or absorptions. This study investigated the diurnal variations of fluxes of three greenhouse gases (CO 2 , CH 4 , and N 2 O) from a mixed broad-leaved and coniferous forest soil. A static chamber-gas chromatograph technique was used to measure the fluxes of three greenhouse gases. By using the improved gas chromatography sampling system, the fluxes were analyzed with a single injection. In order to find out the effects of litter and seedling on the emissions or absorptions of these greenhouse gases, three treatments were set in the field:(1)bare soil surface (litter was removed previously); (3) litter + soil; (3) seedling + litter + soil. The experimental results demonstrated that the forest soil was a source of CO 2 , N 2 O and a weak sink of CH 4 .The daily fluxes of CO 2 , CH 4 , and N 2 O from the soil surface were in the range of 488.99~700.57, 0.049~0.108 and –0.025~ –0.053 mg/(m 2 ·h ), respectively. CO 2 from the litter decomposition accounted for about 1/3 of the total CO 2 emission from the soil surface, while the litter and seedling had no significant effect on the fluxes of CH 4 and N 2 O. The fluxes of CO 2 and N 2 O measured at 9:00 _11:00 a.m. were significantly different from their daily averages. Therefore, caution must be taken if the CO 2 and N 2 O fluxes measured within 9:00_11:00 a.m. are used for extrapolation. The subtropical mixed broad-leaved and coniferous forest, a typical successional monsoon forest, is one of the major forests in the subtropics of China. Therefore, it is very important to estimate the fluxes of the greenhouse gases from the forest soil in order to evaluate the impact of subtropical forests on the greenhouse gas emissions or absorptions. This study investigated the diurnal variations of fluxes of three greenhouse gases (CO 2, CH 4, and N 2 O) from a mixed broad-leaved and coniferous forest soil. A static chamber-gas chromatograph technique was used to measure the fluxes of three greenhouse gases. By using the improved gas chromatography sampling system, the fluxes were analyzed with a single injection. In order to find out the effects of litter and seedling on the emissions or absorptions of these greenhouse gases, three treatments were set in the field: (1) bare soil surface (litter was removed previously); (3) litter + soil; (3) seedling + litter + soil. The experimen tal results demonstrated that the forest soil was a source of CO 2, N 2 O and a weak sink of CH 4. The daily fluxes of CO 2, CH 4, and N 2 O from the soil surface were in the range of 488.99 ~ 700.57, 0.049-0.108, and -0.025-0.053 mg / (m 2 · h), respectively. CO 2 from the litter decomposition accounted for about 1/3 of the total CO 2 emission from the soil surface, while the litter and seedling had no significant effect on the fluxes of CH 4 and N 2 O. The fluxes of CO 2 and N 2 O measured at 9:00 _11: 00 am were significantly different from their daily averages. Therefore, caution must be taken if the CO 2 and N 2 O fluxes measured within 9: 00_11: 00 am are used for extrapolation.