本文报告了应用高效液相色谱法测定冷烫精中的巯基乙酸,本法来用C_8柱,40℃,以甲醇:水=15+85为流动相,检测波长220nm。本法检测下限为0.02mg/ml,至少在0.02～2.0mg/ml范围内呈线性,相关系数r=0.9997,回收率为90.8％～103.5％,变异系数为4.02％,与碘滴定法相比,具有定量准确、迅速、灵敏度高等优点,且二者符合良好。 This paper reports the determination of thioglycolic acid in cold spearmint by high performance liquid chromatography. This method uses C_8 column with methanol: water = 15 + 85 as the mobile phase at 40 ℃ and the detection wavelength is 220nm. The detection limit of this method is 0.02mg / ml, at least in the range of 0.02 ~ 2.0mg / ml linearity, the correlation coefficient r = 0.9997, the recovery rate of 90.8% ~ 103.5%, the coefficient of variation of 4.02% With quantitative accuracy, speed, high sensitivity, and both in good.