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目的:分析并鉴别桑叶水提取物化学成分及其口服后大鼠血清中的成分。方法:采用HPLC对桑叶水提物及灌胃后大鼠血清样品进行分离,用静电场轨道阱组合式高分辨质谱仪进行在线离子检测、二级碎片离子扫描,分析鉴别桑叶水提物体外成分及血中代谢成分。结果:桑叶水提物中初步鉴定了11个化学成分,分别为异绿原酸(1),5,7-二羟基香豆素-7-O-β-D-吡喃葡萄糖苷(2),东莨菪苷(3),绿原酸(4),山柰酚-3,7-二-O-D-吡喃糖苷(5),4-咖啡酰奎宁酸甲酯(6),芦丁(7),金丝桃苷(8),异槲皮苷(9),紫云英苷(10),异鼠李素-3-O-葡萄糖苷(11)。初步鉴定血中成分30个,多为上述苷类的苷元代谢产物,血清中未发现上述11个原型成分。结论:该方法可成功用于桑叶水提物及其口服后血中成分的鉴别,为桑叶进一步的药效研究及二次开发提供了参考。
OBJECTIVE: To analyze and identify the chemical constituents of water extract of mulberry leaves and the components of rat serum after oral administration. Methods: The aqueous extracts of mulberry leaves and the serum samples of rats after gavage were separated by HPLC. The on-line ion detection and the second-level fragment ion scanning were performed by electrostatic field orbit-well combined high-resolution mass spectrometry to identify the aqueous extracts of mulberry leaves External components and blood metabolism. Results: Eleven chemical constituents were identified in the aqueous extracts of mulberry leaves, which were isocyanuric acid (1), 5,7-dihydroxycoumarin-7-O-β-D-glucopyranoside ), Scopolamine (3), chlorogenic acid (4), kaempferol-3,7-di-O-pyranoside (5), methyl 4-caffeoylquinic acid (6) (7), hyperoside (8), isoquercetin (9), orientin (10) and isorhamnetin-3-O-glucoside (11). Preliminary identification of 30 blood components, mostly glycosides metabolites of the above glycosides, the serum did not find the 11 prototype components. Conclusion: The method can be successfully applied to the identification of water extracts of mulberry leaves and its oral components after oral administration, which provides a reference for further research on mulberry leaves efficacy and secondary development.