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目的研究含有IL-21基因的重组腺病毒表达载体(Ad-IL-21)联合γ射线照射对食管癌细胞EC109生长的抑制作用,为肿瘤的基因-放射治疗提供实验依据。方法将Ad-IL-21于体外转染食管癌细胞株EC109,用MTT比色法和流式细胞术观察基因联合照射后EC109细胞的生长曲线和细胞周期的变化。结果 Ad-IL-21基因组、单纯照射组和Ad-IL-21基因联合照射组EC109细胞生长均明显受到抑制,与Ad-lacZ组和空白对照组比较差异均有统计学意义(P<0.05)。其中联合照射组EC109细胞生长最为缓慢,与Ad-IL-21基因组和单纯照射组比较差异均有统计学意义(P<0.05)。Ad-IL-21基因组、单纯照射组和联合治疗组大量的EC109细胞停留在G1期,而G2期和S期细胞明显减少,联合治疗组细胞停留在G1期最多。结论腺病毒介导的白介素21基因转移能显著抑制食管癌EC109细胞的生长,使细胞产生G1期阻滞,与放射治疗联合应用具有相加和协同效应。
Objective To study the inhibitory effect of recombinant adenovirus containing IL-21 gene (Ad-IL-21) combined with γ-ray irradiation on the growth of EC109 esophageal cancer cells and to provide experimental evidence for gene-radiotherapy of tumor. Methods Ad-IL-21 was transfected into esophageal cancer cell line EC109 in vitro. The growth curve and cell cycle of EC109 cells were observed by MTT assay and flow cytometry. Results The growth of EC109 cells was significantly inhibited by combination of Ad-IL-21, irradiation alone and Ad-IL-21. Compared with Ad-lacZ group and blank control group, the difference was statistically significant (P <0.05) . Among them, the EC109 cells in the combination group showed the slowest growth, compared with the Ad-IL-21 group and the simple group (P <0.05). A large number of EC109 cells in the Ad-IL-21 genome, irradiation alone group and combination therapy group remained in the G1 phase, whereas cells in the G2 and S phase were significantly reduced. The cells in the combination therapy group stayed at the G1 phase for the most. Conclusion Adenovirus-mediated interleukin-21 gene transfer can significantly inhibit the growth of EC109 esophageal cancer cells and cause cell arrest in G1 phase, which is additive and synergistic with radiotherapy.