论文部分内容阅读
利用纳秒脉冲电场(nanosecond pulsed electric fields,ns PEFs)治疗肿瘤具有潜在优势。该研究探讨了纳秒脉冲电场对体外培养的人卵巢癌顺铂敏感细胞COC1及顺铂耐药细胞COC1/DDP的生长抑制、凋亡诱导效应及其机制。CCK-8法分析显示,纳秒脉冲电场降低COC1及COC1/DDP细胞存活率,而且抑制效应呈明显时间依赖效应。流式细胞术检测结果结合Hoechst 33342荧光染色结果表明,纳秒脉冲电场可诱导COC1及COC1/DDP细胞发生凋亡。JC-1荧光染色及caspase-3蛋白酶活性检测表明,纳秒脉冲电场处理可引起COC1及COC1/DDP细胞线粒体膜电位降低及caspase-3活性增强。流式细胞术检测细胞周期显示,纳秒脉冲电场可将COC1细胞周期阻滞于S期。以上研究结果揭示,纳秒脉冲电场可以抑制COC1及COC1/DDP细胞增殖,诱导细胞凋亡,线粒体途径凋亡为其可能机制。
The use of nanosecond pulsed electric fields (ns PEFs) to treat tumors has the potential advantage. This study investigated the inhibitory effect of nanosecond pulsed electric fields on the growth inhibition and apoptosis induction of COC1 and cisplatin-resistant cells COC1 / DDP in human ovarian cancer cells cultured in vitro and its mechanism. CCK-8 analysis showed that the nanosecond pulse electric field decreased the survival rate of COC1 and COC1 / DDP cells, and the inhibitory effect was significantly time-dependent. Flow cytometry results combined with Hoechst 33342 fluorescence staining showed that the nanosecond pulse electric field can induce COC1 and COC1 / DDP cells apoptosis. JC-1 fluorescence staining and caspase-3 protease activity assay showed that nanosecond-pulsed electric field treatment could induce mitochondrial membrane potential decrease and caspase-3 activity increase in COC1 and COC1 / DDP cells. Cell cycle analysis by flow cytometry showed that the nanosecond-pulsed electric field could block the COC1 cell cycle in S phase. The above results reveal that nanosecond pulse electric field can inhibit COC1 and COC1 / DDP cell proliferation, induce apoptosis, mitochondrial pathway apoptosis as its possible mechanism.