论文部分内容阅读
目的:制备包裹BSA FITC的纳米乳剂(NEBSA FITC),研究其特性以及树突状细胞(DC)和巨噬细胞对其摄取的效率. 方法:采用界面聚合法,制备包裹BSA FITC的纳米乳剂(NEBSA FITC),于电镜下观察其形态并测量其粒径.用凝胶层析法检 测纳米乳剂的包裹率、载药量及稳定性.将小鼠骨髓DC及腹腔巨噬细胞与NEBSA FITC共育后,用流式细胞仪检测DC及腹腔 巨噬细胞摄取NEBSA FITC的阳性率.用激光共聚焦显微镜观察DC摄取NEBSA FITC的能力.结果:成功地制备出粒径为(25 ±10)nm的纳米乳剂,其包裹率为91%,载药量为0.091g/L,于4℃放置6个月后性质稳定.流式细胞仪检测显示,DC和腹腔 巨噬细胞摄取NEBSA FITC的能力明显强于对游离BSA FITC的摄取.激光共聚焦显微镜下观察到DC可摄取NEBSA FITC. 结论:纳米乳剂具有良好的包裹率和载药量,可有效地增强抗原递呈细胞对所载药物的摄取,有望作为肿瘤抗原疫苗的新型 载体.
OBJECTIVE: To prepare the nanoemulsion (NEBSA FITC) coated with BSA FITC and investigate its characteristics and the efficiency of its uptake by dendritic cells (DCs) and macrophages.METHODS: The nano-emulsion of BSA FITC NEBSA FITC) was used to observe its morphology and measure its particle size under electron microscope.The encapsulation efficiency, drug loading and stability of the nanoemulsion were detected by gel chromatography.Mice bone marrow DC and peritoneal macrophages were co-cultured with NEBSA FITC After incubation, the positive rate of NEBSA FITC uptake by DCs and peritoneal macrophages was detected by flow cytometry.The ability of NEBSA FITC uptake was observed by laser confocal microscopy.Results: The diameter of (25 ± 10) nm Of nanoemulsion with 91% encapsulation efficiency and drug loading of 0.091g / L, which was stable after being stored at 4 ℃ for 6 months.Flow cytometry showed that the ability of DC and peritoneal macrophages to absorb NEBSA FITC was obvious Which is stronger than the uptake of free BSA FITC.Conclusion: DC can absorb NEBSA FITC under confocal laser scanning microscope.Conclusion: Nanoemulsion has a good encapsulation efficiency and drug loading, which can effectively enhance the uptake of drugs by antigen-presenting cells , Is expected as a new type of tumor antigen vaccine Body.