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目的:探讨quercetin对肝癌细胞体内生长的抑制作用及机制。方法:用人肝癌Hep G2细胞皮下接种法建立裸鼠移植瘤模型,成瘤后随机分为对照组、quercetin治疗组、5-FU治疗组、5-FU+quercetin联合治疗组,溶剂或药物均1次/d腹腔注射,3周后观察各组移植瘤大小并计算各治疗组的抑瘤率,分别用RT-PCR、Western blot、免疫组化法检测各组移植瘤组织中cyclin D1、cyclin E、增殖细胞核抗原(PCNA)的表达。结果:与对照组比较,各治疗组移植瘤体积明显减小、移植瘤组织中cyclin D1、cyclin E m RNA和蛋白表达以及PCNA阳性指数均明显降低(均P<0.05),其中联合治疗组的抑瘤率明显大于两个单药组,cyclin D1、cyclin E、PCNA表达的降低程度也明显大于两个单药组(均P<0.05),而两个单药组间各项指标差异均无统计学意义(均P>0.05)。结论:quercetin能通过下调cyclin D1与cyclin E的表达而抑制肝癌细胞的增殖,且与5-FU联合应用具有协同作用。
Objective: To investigate the inhibitory effect of quercetin on the growth of hepatocarcinoma cells and its mechanism. Methods: Nude mice xenografts model was established by subcutaneous inoculation of Hep G2 cells in human hepatoma. The mice were randomly divided into control group, quercetin treatment group, 5-FU treatment group and 5-FU + quercetin combined treatment group The tumor size of each group was observed 3 weeks later, and the tumor inhibition rate of each treatment group was calculated. The expression of cyclin D1 and cyclin E in each group were detected by RT-PCR, Western blot and immunohistochemistry , Proliferating cell nuclear antigen (PCNA) expression. Results: Compared with the control group, the volume of transplanted tumor in each treatment group was significantly reduced, the expression of cyclin D1, cyclin E m RNA and protein and the positive rate of PCNA in the tumor tissue were significantly decreased (all P <0.05) The tumor inhibition rate was significantly higher than that of the two single drug groups, and the reduction of cyclin D1, cyclin E and PCNA expression was also significantly greater than that of the two single drug groups (all P <0.05), but no difference was found between the two single drug groups Statistical significance (all P> 0.05). CONCLUSION: Quercetin can inhibit the proliferation of hepatocellular carcinoma cells by down-regulating the expression of cyclin D1 and cyclin E and has a synergistic effect with 5-FU.