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目的:探讨阿普唑仑(alprazolam,APZ)对PTSD(post traumatic stress disorder)模型大鼠的条件恐惧反应、空间学习记忆能力下降的预防作用。方法:将大鼠随机分为空白对照组、单纯给药组(APZ0.125mg/kg/d,APZ2)、单纯应激组,即单次延长结合足底电击应激(single prolonged stress & foot shock,SPS&S)、应激给药小剂量组(SPS&S+APZ0.06mg/kg/d,SPS&S+APZ1)和大剂量组(SPS&S+APZ0.125mg/kg/d,SPS&S+APZ2)。采用SPS&S动物模型,应激后早期将两种不同浓度的APZ饲喂大鼠14d。第14d在电击箱中检测大鼠的条件性恐惧反应,第15～20d在Morris水迷宫中测大鼠的学习记忆能力。结果:同SPS&S组相比,应激后早期使用APZ干预14d不能改善大鼠空间学习记忆能力的下降趋势,不能显著缩短大鼠上台的潜伏期和增加大鼠在目标象限的时间百分比(%),可以明显缓解应激大鼠的恐惧行为(P<0.05)。结论:应激后早期及时给予APZ干预可明显减轻创伤后应激障碍大鼠的恐惧反应,而对应激造成大鼠的学习记忆能力损伤无缓解作用,这一结果可为临床有效防治创伤后应激障碍提供一条新思路。
AIM: To investigate the preventive effect of alzheimer’s disease (APZ) on conditioned fear reaction (PTSD) and spatial learning and memory in PTSD (post traumatic stress disorder) model rats. Methods: The rats were randomly divided into blank control group, APZ0.125mg / kg / d, APZ2 group, simple stress group, single prolonged stress & foot shock , SPS & S), low dose group (SPS & S + APZ0.06mg / kg / d, SPS & S + APZ1) and high dose group (SPS & S + APZ0.125mg / kg / d, SPS & S + APZ2). Using SPS & S animal model, two different concentrations of APZ were fed to rats 14 days after stress. Rats were subjected to conditioned fear reaction in a shock box on day 14d and learning and memory ability of rats in Morris water maze from the 15th to the 20th day. Results: Compared with SPS & S group, the early use of APZ for 14 d did not improve the spatial learning and memory abilities of rats, but did not significantly reduce the latency of rats and increase the percentage of time (%) in the target quadrant. Can significantly alleviate the fear of stress rats (P <0.05). CONCLUSIONS: APZ intervention given in a timely and early manner after stress can significantly reduce the fear reaction in rats with post-traumatic stress disorder, but does not relieve the learning and memory impairment of rats in response to stress. This result may be clinically effective against trauma Disorder provides a new idea.