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背景:染料木黄酮是植物性雌激素大豆异黄酮的主要成分,其结构与雌激素相似,提示它可能预防或延缓骨质疏松的发生发展。有关染料木黄酮对去势大鼠骨骼矿化及骨钙、磷、锌和锰影响的研究较少。目的:研究染料木黄酮对去势大鼠骨骼矿化及骨钙、磷、锌和锰的影响,为染料木黄酮用于预防骨质疏松提供理论依据。设计:以实验动物为研究对象的对照性实验研究。单位:解放军总医院营养科。材料:实验于2003-02/06在解放军军事医学科学院卫生学环境医学研究所完成。10周龄雌性Wistar大鼠(合格证号:军医动D98014号),体重(170±20)g。干预:实验动物饲正常饲料6周后,改饲AIN-93合成饲料,5d后按体质量随机分为去势组(n=40)和假手术组(n=7)。去势组手术切除双侧卵巢,假手术组只做腹部切开术,恢复5d后,将去势组按体质量随机分为5组,每组8只:去势对照组、雌激素组犤己烯雌酚20μg/(kg·d)犦、染料木黄酮Ⅰ、Ⅱ、Ⅲ组犤剂量分别为25,50,100mg/(kg·d)犦。饲养3个月,随机抽取各组大鼠6只测量骨密度和骨组织形态计量学相关指标。主要观察指标:各组大鼠骨密度、骨矿化相关参数、骨中钙、磷、锌和镁含量。结果:大鼠去势后,股骨骨密度犤(0.247±0.007)g/cm犦降低,平均类2骨质宽度犤(7.04±0.32)μm犦增大,骨矿化延迟时间犤(4.96±0.
BACKGROUND: Genistein is a major component of phytoestrogen soy isoflavones. Its structure is similar to estrogen, suggesting that it may prevent or delay the occurrence and development of osteoporosis. There are few studies on the effects of genistein on skeletal mineralization and calcium, phosphorus, zinc and manganese in ovariectomized rats. OBJECTIVE: To study the effects of genistein on bone mineralization and calcium, phosphorus, zinc and manganese in ovariectomized rats and to provide a theoretical basis for its application in the prevention of osteoporosis. Design: A controlled experimental study of experimental animals. Unit: PLA General Hospital Nutrition. MATERIALS: The experiment was performed at Institute of Hygiene and Environmental Medicine, PLA Academy of Military Medical Sciences from February to February 2003. 10-week-old female Wistar rats (Certificate of Conformity: D98014) and weight (170 ± 20) g. Intervention: The experimental animals were fed with normal diet for 6 weeks and fed with AIN-93 synthetic diet. After 5 days, animals were randomly divided into castration group (n = 40) and sham operation group (n = 7) according to their body weight. The ovariectomized group was surgically removed from the ovary. The sham-operated group was only incised. After 5 days of recovery, the castration group was randomly divided into 5 groups according to body weight: 8 castrated control group, estrogen group Diethylstilbestrol 20μg / (kg · d) 犦, genistein I, Ⅱ, Ⅲ group 犤 doses were 25,50,100 mg / (kg · d) 犦. Feeding for 3 months, six rats from each group were randomly selected to measure bone mineral density and bone histomorphometry related indicators. MAIN OUTCOME MEASURES: BMD, bone mineralization related parameters, contents of calcium, phosphorus, zinc and magnesium in bone in each group. RESULTS: After castration, the BMD of femur decreased (24 0.247 ± 0.007) g / cm, and the mean 2 bone width 犤 (7.04 ± 0.32) μm 犦 increased. The bone mineralization delay time 犤 (4.96 ± 0) .