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目的观察Hp全菌抗原免疫奶羊中特异性抗体产生规律。方法采用菌液浓度为6×109cfuml全菌抗原,通过滴鼻、多点皮下注射、妊娠期肌肉注射三种途径免疫3只产奶山羊。前2组都于0、14、21、28天接受4次免疫接种,妊娠组于分娩前后1个月每隔两周注射1次。收集血清、乳清样本,并以酶联免疫吸附试验进行样本中的IgA、IgG抗体测定。结果3种途径免疫奶羊后都激发了奶羊的系统免疫,产生了血清循环抗体。血清中IgA变化幅度不大,三组血清中IgG较免疫前都有很大升高。羊奶中IgA抗体较免疫前都有升高,且抗体水平滴鼻组>妊娠组>皮下组,羊奶中IgG抗体升高幅度妊娠组>皮下组>滴鼻组。结论3种途径都能激发奶羊机体的免疫应答,使羊奶中抗HpIgA、抗HpIgG抗体水平升高,血清中IgG抗体水平升高,尤其是滴鼻免疫能够诱导机体同时产生不同部位的粘膜免疫和系统免疫,且所需的抗原量远远少于其他两组,是一个敏感、安全、可行有效的免疫途径。
Objective To observe the regularity of the production of specific antibodies in immunized goat with Hp whole-cell antigen. Methods The total bacterial antigen concentration of 6 × 109cfuml bacteria, intranasal injection, multi-point subcutaneous injection, intramuscular injection of three ways pregnant three milk goat. The first two groups received four immunizations on days 0, 14, 21 and 28, and the pregnancy group was injected once every two weeks before and after delivery. Serum and whey samples were collected and IgA and IgG antibodies were measured in the samples by enzyme-linked immunosorbent assay. Results All the three routes of immunization of milk goats stimulated systemic immunity of sheep and produced circulating antibodies. Serum IgA changes in magnitude is not significant, three groups of serum IgG before immunization have greatly increased. IgA antibody in goat milk was higher than before immunization, and the level of antibody in intranasal group> pregnancy group> subcutaneous group and goat’s milk increased IgG antibody in pregnant group> subcutaneous group> intranasal group. Conclusion All three ways can stimulate the immune response of the sheep, so goat milk anti-HpIgA, anti-HpIgG antibody levels increased serum IgG antibody levels, especially intranasal immunization can induce the body at the same time produce different parts of mucosal immunity And immune system, and the required amount of antigen is far less than the other two groups, is a sensitive, safe, viable and effective immunization pathway.