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[目的]了解表皮生长因子受体(EGFR)信号通路和microRNA(miRNA)表达的关系,探讨miRNA在肺癌转移中的作用。[方法]采用miRNA芯片分析表皮生长因子(EGF)刺激前后肺癌细胞中miRNA表达谱的改变;利用Transwell Insert迁移与侵袭实验,体外划痕愈合实验了解miR-125a-5p抑制肺癌细胞迁移与侵袭的能力;微管形成实验了解脐静脉血管内皮细胞(ECV304)体外形成血管的能力。[结果]芯片检测与RT-PCR显示34个miRNA的表达受到EGFR信号通路活化的影响,其中miR-125a-5p改变最明显;转染miR-125a-5p抑制物后PC9细胞的迁移数(286.67±10.51 vs 218.00±9.54,P=0.001),侵袭数(245.67±5.51 vs 186.33±5.03,P<0.001),创伤愈合能力(P<0.05)较阴性对照增加,ECV304细胞形成的微管数量也增多。[结论]miR-125a-5p可能受EGFR信号通路调节,并且对肺癌细胞具有转移抑制作用。
[Objective] To investigate the relationship between epidermal growth factor receptor (EGFR) signaling pathway and the expression of microRNA (miRNA), and explore the role of miRNA in lung cancer metastasis. [Methods] The expression of miRNA in lung cancer cells before and after epidermal growth factor (EGF) stimulation was analyzed by miRNA microarray. Transwell Insert migration and invasion assay were used to detect the wound healing in vitro to understand the role of miR-125a-5p in inhibiting lung cancer cell migration and invasion Ability; Microtubule formation assay to understand the ability of umbilical vein endothelial cells (ECV304) to form blood vessels in vitro. [Results] The results of microarray detection and RT-PCR showed that the expression of 34 miRNAs was affected by the activation of EGFR signaling pathway, of which miR-125a-5p had the most obvious change. The migration of PC9 cells after transfection with miR-125a-5p inhibitor (286.67 (P <0.001), and the invasion number (245.67 ± 5.51 vs 186.33 ± 5.03, P <0.001), the wound healing ability (P <0.05) increased compared with the negative control group, and the number of microtubules formed by ECV304 cells also increased . [Conclusion] miR-125a-5p may be regulated by EGFR signaling pathway and has metastasis-inhibiting effect on lung cancer cells.