目的 :探讨外源性CO对大鼠肺动脉平滑肌细胞迁移的影响。方法 :体外培养大鼠肺动脉平滑肌细胞 ,用外源性CO处理 2 4h、48h、72h ,检测平滑肌细胞迁移情况。结果 :2 4h、48h、和 72h后未处理的对照组大鼠肺动脉平滑肌细胞迁移距离分别为 ( 332 16± 49 94)nm、( 44 2 83± 5 9 15 )nm和 ( 5 94.92± 6 2 .5 3)nm、后二者与 2 4h组比有显著差异 (P <0 .0 0 1)。 2 0、5 0、80 ppmCO处理细胞 2 4h后 ,细胞迁移距离分别为 ( 2 96 13± 5 5 32 )nm、( 2 47.87±5 8.2 3)nm和 ( 2 2 8 5 7± 72 6 8)nm ,除 2 0ppmCO组与对照组无显著差异 (P <0 .0 5 ) ,其余两组均显著低于对照组(P <0 .0 1)。 2 0、5 0、80 ppmCO处理细胞 48h、72h后 ,其迁移距离也显著低于对照组 (P <0 .0 1)。 结论 :大鼠肺动脉平滑肌细胞自身存在迁移情况 ,培养时间愈长 ,迁移距离愈大 ;外源性CO可以显著抑制平滑肌细胞的迁移 ,随着浓度增加 ,抑制作用增强 Objective: To investigate the effect of exogenous CO on pulmonary artery smooth muscle cell migration in rats. Methods: Pulmonary artery smooth muscle cells were cultured in vitro and treated with exogenous CO for 24 hours, 48 ​​hours and 72 hours to detect the migration of smooth muscle cells. RESULTS: After 2, 4, 48, and 72 h, the migration of pulmonary artery smooth muscle cells in untreated control rats was (332 16 ± 49 94) nm, (44 2 83 ± 5 9 15) nm and (5 94.92 ± 62) .5 3) nm, there was a significant difference between the latter two groups and the 24 h group (P <0.01). The cell migration distance was (2 96 13 ± 5 5 32) nm, (2 47.87 ± 5 8.2 3) nm and (2 2 8 5 7 ± 72 6 8) respectively after the cells were treated with 0, ) nm, except 20ppmCO group had no significant difference with the control group (P <0.05), the other two groups were significantly lower than the control group (P <0.01). After treated with 0, 50, 80 ppmCO for 48h, the migration distance was also significantly lower than that of the control group after 72h (P <0.01). CONCLUSION: The pulmonary artery smooth muscle cells of rats have their own migration, the longer the culture time, the larger the migration distance. Exogenous CO can significantly inhibit the migration of smooth muscle cells. With the increase of concentration, the inhibitory effect is enhanced