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目的：探讨肿瘤坏死因子－α（ＴＮＦ－α）与慢性阻塞性肺疾病（ＣＯＰＤ）的发病关系和临床意义。方法：采用酶联免疫吸附法（ＥＬＩＳＡ）检测１０例慢性阻塞性肺疾病急性发作期及１０例正常人痰和血清的ＴＮＦ－α水平。结果：慢性阻塞性肺疾病急性发作期痰中的ＴＮＦ－α水平［（９８．０±２０．５）ｎｇ／Ｌ］明显高于血清［（１２．５±４．０）ｎｇ／Ｌ］和正常对照组痰液［（２２．０±６．８）ｎｇ／Ｌ］（Ｐ＜０．０５）。而且ＣＯＰＤ痰中ＴＮＦ－α水平与ＦＥＶ１呈明显负相关（ｒ＝－０．４５，Ｐ＜０．０５）。结论：ＴＮＦ－α在ＣＯＰＤ气道炎症及气道阻塞的形成的机制上有重要作用，痰液检测ＴＮＦ－α含量较血清更能直接反映气道炎症轻重
Objective: To investigate the relationship between tumor necrosis factor-α (TNF-α) and chronic obstructive pulmonary disease (COPD) and its clinical significance. Methods: The levels of TNF-α in 10 cases of chronic obstructive pulmonary disease and 10 cases of normal sputum and serum were detected by enzyme linked immunosorbent assay (ELISA). Results: The levels of TNF-α in the sputum of patients with chronic obstructive pulmonary disease ([(98.0 ± 20.5) ng / L] were significantly higher than those in serum [(12.5 ± 4.0) ng / L Normal control group sputum [(22.0 ± 6.8) ng / L] (P <0.05). Moreover, the level of TNF-α in sputum of COPD was negatively correlated with FEV1 (r = -0.45, P <0.05). Conclusion: TNF-α plays an important role in the pathogenesis of airway inflammation and airway obstruction in COPD. The detection of TNF-α in sputum can reflect the severity of airway inflammation more directly than serum