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利用高效持续带毒增殖的细胞株传代增殖获取甲型肝炎病毒（ Ｈ Ａ Ｖ） 。采用不连续蔗糖／甘油密度梯度超速离心分离高度纯化的 Ｈ Ａ Ｖ 颗粒。温和病毒裂解液裂解病毒后，运用高效液相色谱分离出三种蛋白， Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 和 Ｗｅｓｔｅｒｎｂｌｏｔ 证实所分离蛋白为 Ｈ Ａ Ｖ 三种主要结构蛋白 Ｖ Ｐ１ 、 Ｖ Ｐ２ 和 Ｖ Ｐ３ ，蛋白含量较高
Hepatitis A virus (H A V) was obtained by passage and proliferation of cell strains highly efficient and sustained. Highly purified HAV particles were isolated by discontinuous sucrose / glycerol density gradient ultracentrifugation. After mild virus lysis solution, the three proteins were separated by high performance liquid chromatography (HPLC). SDS-PAGE and Western-blot showed that the isolated proteins were H A V three major structural proteins V P1, V P2 and V P3, high protein content