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设计结合菌苗以优化其免疫学特性尚未被广泛研究,应用包含末端活化的多糖片段的结合物的合成,就可非常有效地分析结合物特异结构参数改变与免疫学特性的关系.结合前,随机活化多糖片段可产生更复杂的未定结合物.当多糖-蛋白结合物中多糖片段长度发生改变时更是如此。糖片段的抗原性和免疫原性均依赖于长度,抗原性与小于抗体位点的寡糖表位有关.然而,新近识别的长度稳定的结构象糖表位给这一概念增添了新内容.多糖-蛋白结合物的免疫学特性亦具有长度依赖性,优化免疫学特性可能需要多个表位.
The design of a bacterin to optimize its immunological properties has not been extensively studied and the use of a conjugate comprising a polysaccharide fragment with an end activation can be very effective in analyzing the relationship between changes in the specific structural parameters of a conjugate and the immunological properties, Random activation of polysaccharide fragments produces more complex undefined conjugates, especially when the length of the polysaccharide fragment in the polysaccharide-protein conjugate changes. The antigenicity and immunogenicity of the carbohydrate fragments are both length dependent and antigenicity is related to oligosaccharide epitopes that are smaller than the antibody site. However, newly identified structures of length-stable carbohydrate epitopes add a new dimension to this concept. The immunological properties of polysaccharide-protein conjugates are also length dependent, and optimizing immunological properties may require multiple epitopes.