在聚脂纤维膜和普通载物片上,运用氩离子激光束对大麦的染色体进行显微分割。光束的强度和位置由微型计算机控制。对染色体片段分割的最适激光强度通过实验得以确定。结果表明:大麦和水稻染色体的着丝点、随体、短臂和长臂等特定区域,可从分散的染色体样本中分割出来,并且成功地将该特定染色体片段转移到Eppendorf管中。 The chromosomes of barley were microdifferentiated using an argon ion laser beam on a polyester film and a conventional slide. The intensity and position of the beam are controlled by a microcomputer. The optimal laser intensity for chromosome segment segmentation was experimentally determined. The results showed that the centromeric dots of barley and rice chromosomes could be segregated from scattered chromosome samples with specific regions such as body, short arm and long arm, and the specific chromosome segments were successfully transferred into Eppendorf tubes.