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目的建立稳定的系统性念珠菌感染小鼠动物模型。方法将健康雄性昆明小鼠随机分成3组,实验组1经鼠尾静脉注射相同数量的对数生长期白念珠菌酵母细胞(2×107CFU/mL,0.5mL),实验组2经鼠尾静脉注射相同数量的对数生长期白念珠菌酵母细胞(2×106CFU/mL,0.5mL),正常对照组注射0.5mL的PBS磷酸盐缓冲液。进行小鼠死亡率观察;小鼠脾脏组织白念珠菌培养、鉴定;将肾脏组织做病理学标本PAS染色。结果实验组小鼠的死亡率为100%,高浓度组3天内全部死亡,低浓度组生存期稍长,但观察期间也全部死亡;两实验组脾组织培养有白念珠菌生长;肾组织病理学检查发现炎性肉芽肿形成,肉芽肿内有大量的白念珠菌孢子和菌丝生长及炎细胞浸润。结论直接将适量的白念珠菌酵母细胞经尾静脉注射入小鼠体内可以建立稳定的系统性念珠菌病小鼠模型,避免了应用免疫抑制剂所导致的人为性实验干扰因素,更好地为系统性念珠菌病的研究提供了动物模型。
Objective To establish a stable animal model of systemic candidal infection in mice. Methods Healthy male Kunming mice were randomly divided into 3 groups. In the experimental group 1, the same number of logarithmic growth phase Candida albicans yeast cells (2 × 107CFU / mL, 0.5mL) were injected into the tail vein of mice in experimental group 2, The same number of log phase Candida albicans yeast cells (2 × 10 6 CFU / mL, 0.5 mL) were injected, and the normal control group was injected with 0.5 mL of PBS phosphate buffered saline. The mice were observed for mortality; Candida albicans was cultured and identified in the spleen of mice; PAS was used to stain the kidney tissue in pathological specimens. Results The death rate of mice in the experimental group was 100%. All the mice in the high concentration group died within 3 days. The survival rate in the low concentration group was slightly longer, but all the mice died during the observation period. Candida albicans grew in the spleen tissue of two experimental groups, Neutropenia found granulomas, granulomas within a large number of Candida albicans and mycelial growth and inflammatory cell infiltration. Conclusions Direct injection of proper amount of Candida albicans yeast cells into mice through tail vein can establish a stable mouse model of systemic candidosis and avoid the interference factors of artificial experiment caused by immunosuppressive agents, Studies of systemic candidiasis provide animal models.