论文部分内容阅读
作者以灰盖鬼伞为材料,选择具有亲和性的AB二的二倍体单核孢子进行交配,从遗传学和细胞学方面都证实得到了四倍体子实体(2n+2n)。尽管四倍体是同质四倍体,但和已报道过的三倍性子实体一样,孢子形成不好。在光学镜和电镜下,与正常二倍体进行比较观察,结果如下:于30℃下培养,经2小时光照,二倍体的担子中,大部分合子核融合完毕,并进入减数分裂Ⅰ期,而四倍体仍处于核融合阶段。此后,二倍体子实体减数分裂Ⅰ前期持续6小时,担子形成大致是同步进行,而四倍体子实体光照9小时,多数仍处于减数分裂Ⅰ前期,像三倍体细胞所见到的减数分裂延迟一样,四倍体细胞空泡化致使细胞质崩溃。
In this paper, we selected the diploid mononuclear spores of AB II with affinity for mating, and confirmed the tetraploid fruiting body (2n + 2n) genetically and cytologically. Although tetraploids are homogeneous tetraploids, spore formation is poor, as has been reported for triploids. Under the light microscope and electron microscope, compared with the normal diploid, the results were as follows: After culturing at 30 ℃ for 2 hours, most of the zygotic nuclei in diploid burden were fused and entered meiosis Ⅰ While tetraploids are still in the stage of nuclear fusion. Since then, the diploid fruit body meiosis Ⅰ pre-last 6 hours, the burden is formed roughly synchronized, and tetraploid fruit body illumination 9 hours, most are still in the meiosis pre-I, as seen in the triploid cells As with the meiosis delay, tetraploid cells cause vacuolization of the cytoplasm.