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[目的]西花蓟马Frankliniella occidentalis(Pergande)是一种重要的外来有害生物,已在我国多个省份发现危害,且有向南北两端继续扩张的趋势.HSP70是热激蛋白家族中最重要的一类成员,在昆虫温度耐受性中发挥重要的作用.本研究旨在明确不同性别的西花蓟马成虫在受到高低温胁迫时其HSP70家族的表达差异,进一步探讨西花蓟马对环境适应的分子机理,为其防控提供理论依据.[方法]首先,应用RT-PCR及RACE技术从西花蓟马中克隆得到HSP70基因;其次,对该基因组进行验证,得到基因组序列,并分析内含子的位置及大小;最后,通过实时定量PCR技术分析得到的西花蓟马HSP70的表达模式.[结果]本研究克隆得到的西花蓟马HSP70基因命名为Fohsp703(GenBank登录号:KY914546),该基因全长2106 bp,开放阅读框2040 bp,编码679个氨基酸,推测分子量为73.6 ku.FoHSP703的氨基酸序列中含有3个HSP70家族签名序列,说明它是热激蛋白家族成员;但是,FoHSP703的蛋白序列以APAA结尾,而且多序列比对与系统发育分析均表明,FoHSP703不同于现在已鉴定出的其它昆虫的HSP70.基因组结构分析发现,Fohsp703不含有内含子,在高低温胁迫下能够被诱导并且显著表达,而且雌虫的表达量高于雄虫,分别在37℃和﹣12℃达到最大值.[结论]本研究的西花蓟马HSP70(FoHSP703)是一种新的昆虫热诱导型HSP70,在西花蓟马温度耐受性中起着重要的作用.“,”[Objectives] In recent years, the Western flower thrip, Frankliniella occidentalis (Pergande), has been found in several Chinese provinces and the range of this pest appears to be expanding in China. Heat shock protein 70 (HSP70) is one of the most important members of the heat shock protein family and plays a very important role in thermotolerance in insects. The aim of this study was to clarify differences in the expression of HSP70 in adult male and female F. occidentalis that were subject to temperature stress, both to further explore the molecular mechanism by which this species responds to temperature stress, and to provide a theoretical basis for the control of this pest. [Methods] The Western flower thrip HSP70 gene was cloned using RT-PCR and RACE. The genomic sequence was then determined by genomic validation, and the position and size of the intron were analyzed by cDNA sequence analysis. Finally, Real-time PCR was used to analyze the expression patterns of HSP70. [Results] The HSP70 gene obtained was named Fohsp703 (GenBank accession number: KY914546) which had a complete cDNA sequence of 2106 bp in length, an open reading frame (ORF) of 2040 bp and encoded 679 amino acids with a predicted molecular weight of 73.6 ku. Its amino acid sequence contains three HSP70 family signature sequences, indicating that HSP70 is a member of the heat shock protein family. However, unlike the HSP70 genes of other species, the Fohsp703 protein sequence ends with APAA, and phylogenetic analysis also suggests that Fohsp703 differs from the HSP70 genes of other insects. Analysis of its genomic structure revealed that Fohsp703 does not contain introns. The expression of Fohsp703 was significantly elevated by heat and cold stress. Expression was higher in adult females than in males, peaking at 37 and℃ ﹣12 , respectively.℃ [Conclusion] The Western flower thrip HSP703 gene is a novel member of the insect HSP70 family and plays an important role in resistance to extreme temperatures.