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目的探讨实验性癫痫持续状态(SE)对大鼠认知功能的影响及与大鼠海马CA1、CA3齿状回超微结构改变的关系。方法30只大鼠随机分为SE组和对照组,戊四氮诱导大鼠SE,采用抬高迷宫和Morris水迷宫试验观察大鼠情感反应和学习记忆功能的改变。电镜观察大鼠海马CA1、CA3区齿状回超微结构改变。结果SE组大鼠在抬高迷宫开放臂中逃避时间延长(P<0.01),进入次数增多(P<0.01);水迷宫中逃避潜伏期延长(P<0.01),搜寻策略改变(P<0.05),平台象限游泳时间百分比降低(P<0.01),穿越平台次数减少(P<0.01)。大鼠海马CA3区和齿状回分子层的神经元胞体固缩浓染,线粒体嵴局部消失,突起膜发生断裂,髓鞘板层松散,突触前后膜结构模糊,突触小泡减少,线粒体空化,星形胶质细胞高度水肿液化,核固缩。结论SE使大鼠情感行为发生改变和学习记忆功能受损,可能与海马超微结构的改变有关。
Objective To investigate the effect of experimental status epilepticus (SE) on cognitive function in rats and the relationship with changes of dentate gyrus ultrastructure in hippocampal CA1 and CA3 of rats. Methods Thirty rats were randomly divided into SE group and control group. Pentylenetetrazol induced SE in rats. The elevation of maze and Morris water maze test were used to observe the changes of affective response and learning and memory in rats. Electron microscope observation of hippocampal CA1, CA3 area dentate gyrus ultrastructural changes. Results In SE group, the evasion time was prolonged (P <0.01) and the number of entry was increased (P <0.01). The escape latency was prolonged in water maze (P <0.01) and the search strategy was changed (P <0.05) , The percentage of swimming time in the platform quadrant decreased (P <0.01), and the number of crossing the platform decreased (P <0.01). In rat hippocampal CA3 region and dentate gyrus, the neuronal somatic cell condensed and stained, the mitochondrial cristae disappeared locally, the rupture of the protuberant membrane, the loose layer of myelin sheath, the synaptic structure and synaptic vesicle diminished, mitochondria Cavitation, astrocyte highly edema liquefaction, nuclear pyknosis. Conclusion SE can change the affective behavior and impairment of learning and memory in rats, which may be related to the changes of ultrastructure in hippocampus.