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目的：通过检测洗精培养液和受精培养液中活性氧的关系，探讨精卵孵育不同时间对体外受精-胚胎移植（IVF-ET）结局的影响。方法接受常规体外受精（IVF）受精的夫妇161例，精液经处理后留取标本（即洗精培养液）3份，分别定为加精后0h、5h和20h检测过氧化氢（H2O2）、过氧化氢酶（CTA）。每名患者取出的卵冠丘复合物（OCCCs）随机分为两组：A组为短时受精，B组为长时受精，检测移卵后的受精培养液中H2O2、CTA的含量。移植的123名患者随机分成两组，一组62人选择短时受精的胚胎进行移植，另一组61人选择长时受精的胚胎进行移植。结果 H2O2及CTA水平在授精后5h明显与A组呈正相关（r值分别为0.477和0.518，P＜0.05），在授精后20h与B组呈明显正相关（r值分别为0.681和0.868，P＜0.05）。授精后5h及授精后20h的洗精培养液中H2O2水平均明显高于相应时间的受精培养液（P＜0.05），而CTA水平只在授精后5h明显低于A组的受精培养液（P＜0.05），在授精后20h与B组之间没有明显差异（P＞0.05）。B组的多精受精率明显高于A组（P＜0.05），A组的优胚率明显高于B组（P＜0.05），两组间的受精率、卵裂率、植入率、临床妊娠率无统计学差异。结论缩短精卵孵育时间可以使卵子尽早脱离高活性氧的环境，有利于胚胎的发育。“,”Objective To analyze the relationship of reactive oxygen species between the sperm wash medium and insemination medium, and explore the effect of different incubation time on the outcome of in vitro fertilization and embryo transfer. Methods One hundred and sixty-one patients who underwent in vitro fertilization and embryo transfer for female infertility were recruited in the study. Three samples were collected after the semen of patients was pretreated with Pure Sperm. Each was defined as 0h after insemination, 5h after insemination and 20h after insemination to detect of the content of H2O2 and CTA. On the oocyte retrieval day, the OCCCs which retrieved from the same patient were divided into short-term fertilization group (group A) and long-term fertilization group (group B) according to incubation time. The concentrations of H2O2 and CTA in insemination medium were detected respectively. On the embryo transfer day,the patients were divided into two groups: one group of patients chose short-term fertilized embryo to transfer, one group of patients chose long fertilized embryo to transfer. Results The levels of hydrogen peroxide and catalase at 5h after insemination were positively correlated with those of group A(r=0.477, 0.518, P<0.05), and the levels of hydrogen peroxide and catalase at 20h after insemination were positively correlated with those of group B(r=0.681, 0.868, P<0.05). The levels of hydrogen peroxide at 5h, 20h after insemination were corresponding higher than that in the insemination medium of group A and group B significantly (P<0.05). The levels of catalase at 5h after insemination were lower than those in the insemination medium of group A significantly (P0.05). The polyspermy rate in group B was significantly higher than that in group A(P<0.05), and the high quality embryo rate in group A was significantly higher than that in group B(P0.05). Conclusion Shorten the incubation time of oocytes and spermcould protect oocytes from the high reactive oxygen environment so as to improve the quality of embryos.