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目的：探讨检测鼻咽癌的酶联免疫吸附（ＥＬＩＳＡ）方法。方法：以正丁酸和巴豆油诱导Ｒａｊｉ细胞表达的ＥＢ病毒早期蛋白为抗原，用ＥＬＩＳＡ方法检测血清中抗ＥＢ病毒相关早期蛋白的ＩｇＧ抗体水平（吸光度Ａ４９０）。其中鼻咽癌４５４例，肝癌４０例，肺癌２３例，肠癌２０例，乳腺癌２３例，卵巢癌１６例，头颈肿瘤１５例和健康人５２４例。结果：在所有被检血清中鼻咽癌血清Ａ值最高（０．３３３±０．１００），（Ｐ＜０．００１）。以正常人９５％特异性Ａ４９０＜０．１８为界，ＥＬＩＳＡ方法诊断鼻咽癌的敏感性为８９％（４０５／４５４）特异性为９４％（４９３／５２４）。结论：用来自Ｒａｊｉ细胞内的ＥＢ病毒早期蛋白为抗原建立的ＥＬＩＳＡ方法可以应用于鼻咽癌的血清学诊断。
Objective: To explore the method of enzyme-linked immunosorbent assay (ELISA) for detecting nasopharyngeal carcinoma. METHODS: The EB virus early protein expressed in Raji cells was induced with n-butyric acid and croton oil as antigen, and the IgG antibody level (absorbance A490) of anti-EB virus-related early proteins was detected by ELISA. There were 454 cases of nasopharyngeal carcinoma, 40 cases of hepatocellular carcinoma, 23 cases of lung cancer, 20 cases of intestinal cancer, 23 cases of breast cancer, 16 cases of ovarian cancer, 15 cases of head and neck tumors, and 524 cases of healthy persons. RESULTS: The serum A value of nasopharyngeal carcinoma was highest in all tested sera (0.333±0.100) (P<0.001). Diagnosing nasopharyngeal carcinoma with sensitivity of 89% (405/454) and specificity of 94% (493/524) based on the 95% specific A490<0.18 of normal subjects. Conclusion: The ELISA method established with the early EBV protein from Raji cells as antigen can be used for serological diagnosis of nasopharyngeal carcinoma.