论文部分内容阅读
目的 研究外源维生素 C(VC)和谷胱甘肽 (GSH)对家兔离体肺泡巨噬细胞(AM)超微弱 (自主 )发光的影响。方法 将 AM悬浮在含有 VC或 GSH的 DMEM培养介质中 ,放入一特制恒温箱的培养盒内 ,连续通入不同浓度的氧气 ,用发光仪检测培养 AM的超微弱发光。结果 浓度超过 0 .3mmol/L的 VC能明显增强有氧培养细胞超微弱发光和加速细胞死亡 ,对无氧培养细胞的影响不明显。低浓度 (0 .0 3mmol/L) VC与 GSH相同 ,能降低由高浓度 (99.1 % )氧暴露引起的细胞发光增强。结论 细胞在含氧气体中的自发氧化是产生超微弱发光的重要原因 ;高浓度 VC能促进离体细胞氧化损伤 ,GSH则有抗氧化作用。
Objective To investigate the effects of exogenous vitamin C (VC) and glutathione (GSH) on the ultraweak (autonomous) luminescence of isolated alveolar macrophages (AM) in rabbits. Methods AM was suspended in DMEM medium containing VC or GSH and placed in a special incubator. Different concentrations of oxygen were continuously introduced into the culture medium, and the ultramicron luminescence of AM was detected by luminometer. Results The concentration of VC above 0.3 mmol / L could significantly increase the ultra-weak luminescence and accelerate the cell death in aerobic cultured cells, and had no obvious effect on the cultured cells in anaerobic conditions. Low concentrations (0.3 mmol / L) of VC, like GSH, reduced luminescence enhancement caused by high concentrations of oxygen (99.1%) exposure. CONCLUSIONS: Spontaneous oxidation of cells in oxygen-containing gas is an important cause of ultraweak luminescence. High concentrations of VC can promote oxidative damage in vitro and GSH has anti-oxidative effects.