研究了ｒｈＧＭＣＳＦ／ＩＬ３融合蛋白对人骨髓造血祖细胞（ＣＦＵＧＭ、ＣＦＵＧＥＭＭ、ＢＦＵＥ）集落形成的影响，结果表明ｒｈＧＭＣＳＦ／ＩＬ３能显著促进ＣＦＵＧＭ、ＣＦＵＧＥＭＭ、ＢＦＵＥ集落的形成，分别与ＧＭＣＳＦ、ＩＬ３单独或联合用药比较，差异有显著性（Ｐ＜０００１），ＣＦＵＧＭ、ＣＦＵＧＥＭＭ、ＢＦＵＥ集落的形成对融合蛋白均有剂量依赖性，培养体系浓度在５～１０ｎｇ／ｍｌ时，各个集落生长效果最好。提示ｒｈＧＭＣＳＦ／ＩＬ３对造血祖细胞的生长发挥着重要作用，可能成为放疗、化疗后继ＧＭＣＳＦ及ＩＬ３更重要的造血制剂。 The effect of rhGMCSF / IL3 fusion protein on the colony formation of human bone marrow hematopoietic progenitor cells (CFUGM, CFUGEMM, BFUE) was studied. The results showed that rhGMCSF / IL3 could significantly promote CFUGM , CFUGEMM, BFUE colony formation, respectively, compared with GMCSF, IL3 alone or in combination, the difference was significant (P <0001), CFU  GM, CFU  GMEM, BFU  E The formation of colonies was dose-dependent on the fusion proteins. When the concentration of culture system was 5 ~ 10ng / ml, the colony growth was the best. Tip rhGM  CSF / IL  3 on the growth of hematopoietic progenitor cells play an important role, may become radiotherapy, chemotherapy followed by GM  CSF and IL  3 more important hematopoietic preparations.