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目的检测胰腺癌中p15、p16基因CpG岛的异常甲基化情况,分析其与胰腺癌发生、发展的关系;探索其在临床早期基因诊断和治疗中的意义以及与胰腺癌病理生理特征的相关性。方法运用甲基化特异性PCR(Methylation-specificPCR,MSP),对29例胰腺癌和3例慢性胰腺炎的石蜡组织、2例正常肝组织、以及12例正常成人外周血白细胞DNA的p15、p16抑癌基因启动子区CpG岛甲基化状态进行了检测。结果对照组p15、p16基因CpG岛甲基化扩增均呈阴性,非甲基化扩增均阳性。胰腺癌中p16、p15基因CpG岛甲基化阳性率分别为37.9%和27.5%,p16、p15基因CpG岛甲基化总检出阳性率为44.8%。癌组织中同时存在p16、p15基因CpG岛异常甲基化为6例。结论胰腺癌p15、p16基因CpG岛甲基化是早期事件,可试作为早期基因诊断的分子标志。p16、p15基因CpG岛异常甲基化,同病人的病理特征无明显的相关关系。
Objective To detect the abnormal methylation status of CpG islands of p15 and p16 genes in pancreatic cancer and to analyze their relationship with the occurrence and development of pancreatic cancer and to explore their significance in the early diagnosis and treatment of clinical genetics and the pathophysiological characteristics of pancreatic cancer Sex. Methods Methylation-specific PCR (MSP) was used to detect the expression of p15, p16 in 29 cases of pancreatic cancer and 3 cases of chronic pancreatitis paraffin, 2 cases of normal liver tissues, and 12 cases of normal adult peripheral blood leukocyte DNA The methylation status of CpG island in tumor suppressor gene promoter region was detected. Results CpG island methylation amplification of p15 and p16 genes in the control group were all negative, and non-methylation amplification was positive. The positive rates of CpG island methylation of p16 and p15 genes in pancreatic cancer were 37.9% and 27.5% respectively, and the positive rates of CpG island methylation in p16 and p15 genes were 44.8%. The abnormal methylation of CpG island in p16 and p15 genes was found in 6 cases. Conclusion CpG island methylation of p15 and p16 genes in pancreatic cancer is an early event that may serve as a molecular marker for early genetic diagnosis. The abnormal methylation of p16 and p15 CpG island showed no significant correlation with the pathological features of the patients.