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目的:探讨前列腺癌微环境中癌相关成纤维细胞(carcinorna-associated fibroblasts,CAFs)对非雄激素依赖性前列腺癌细胞株Du-145生物学行为的影响。方法:原代培养前列腺癌CAFs并制备CAFs条件培养基(CAFs-CM),通过增殖实验、平板集落形成实验、侵袭实验等体外实验,观察CAFs对前列腺癌细胞生物学行为的影响。结果:由从人新鲜前列腺癌组织中分离培养CAFs制备的CAFs-CM剂量依赖性地提高了Du-145细胞的增殖能力,与SFM相比,0.5 g/L的CAFs-CM明显增强了Du-145细胞的增殖能力,两者比较差异有统计学意义[(290.6±25.2)vs(88.4±12.1),P<0.05],其细胞增殖指数Ki-67明显提高(80%vs 20%);在CAFs-CM中培养的Du-145细胞与在DMEM中培养的细胞相比,其平板形成集落的能力明显提高,差异有统计学意义[(0.42±0.09)vs(0.23±0.03),P<0.05];在CAFs-CM中培养的前列腺癌Du-145细胞的变形迁徙破坏能力比在DMEM中培养的Du-145细胞明显增强[(162.12±9.13)vs(112.34±8.45),P<0.01]。结论:前列腺癌间质微环境中的CAFs促进了前列腺癌细胞的浸润与转移,是抗前列腺癌治疗药物设计的新靶点。
Objective: To investigate the effect of cancer-associated fibroblasts (CAFs) on the biological behavior of androgen-independent prostate cancer cell line Du-145 in prostate cancer microenvironment. Methods: Primary CAFs were cultured and CAFs-conditioned media (CAFs-CM) were prepared. The proliferation and proliferation of prostate cancer cells were observed by in vitro experiments. Results: CAFs-CM prepared from CAFs isolated from human fresh prostate cancer tissues increased dose-dependently the proliferation of Du-145 cells. Compared with SFM, CAFs-CM at 0.5 g / L significantly enhanced Du- 145 cells, the difference was statistically significant ([(290.6 ± 25.2 vs 88.4 ± 12.1, P <0.05], and the cell proliferation index Ki-67 was significantly increased (80% vs 20% The ability of Du-145 cells cultured in CAFs-CM to form colonies in DMEM was significantly higher than that in DMEM [(0.42 ± 0.09) vs (0.23 ± 0.03) P <0.05 ]. The ability of migration and destruction of Du-145 cells cultured in CAFs-CM was significantly higher than that of Du-145 cells cultured in DMEM [(162.12 ± 9.13) vs (112.34 ± 8.45, P <0.01]. Conclusions: CAFs in prostate stromal microenvironment promote the invasion and metastasis of prostate cancer cells, which is a new target for anti-prostate cancer drug design.