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目的探讨洛铂对肝癌细胞的放疗增敏效应。方法用MTT法确定洛铂对肝癌细胞株HepG2半数抑制浓度(IC50),流式细胞仪检测1/4IC50、1/8IC50洛铂对HepG2细胞周期、凋亡的影响,蛋白免疫印迹检测凋亡相关蛋白Bcl-2、Bax、Caspase-3的表达,细胞克隆形成实验研究洛铂对HepG2的放射增敏比。结果洛铂对肝癌细胞株HepG2细胞毒作用呈剂量依赖性,半数抑制浓度为1.85μg/ml,不同浓度洛铂组(1/4IC50组、1/8IC50组)均观察到HepG2细胞凋亡和G2/M期阻滞明显增加(P<0.05);两组洛铂干预组肝癌细胞Bcl-2表达下降,Bax、Caspase-3表达水平升高;洛铂对肝癌细胞HepG2的放疗增敏比分别是1.12、1.28。结论洛铂具有放射增敏作用;其机制可能与促进肝癌细胞凋亡以及细胞G2/M期阻滞相关。
Objective To investigate the radiosensitizing effect of lobaplatin on hepatoma cells. Methods MTT assay was used to determine the inhibitory concentration (IC50) of HepG2 cells treated with Losoplatin. The cell cycle and apoptosis of HepG2 cells treated with 1 / 4IC50,1 / 8IC50 and flow cytometry were detected by flow cytometry. Apoptosis was detected by Western blotting The protein Bcl-2, Bax, Caspase-3 expression, cell clone formation experiments to study the radiosensitization ratio of lobaplatin to HepG2. Results The inhibitory effect of lobaplatin on HepG2 cells was dose-dependent and the half-inhibitory concentration was 1.85μg / ml. The apoptosis of HepG2 cells was observed in different concentrations of lobaplatin group (1 / 4IC50, 1/8IC50) (P <0.05). The Bcl-2 expression and the expression of Bax and Caspase-3 in HepG2 cells were significantly decreased in the two groups treated with Losplatin, and the sensitivities of radiotherapy to HepG2 were 1.12,1.28. Conclusion Losoplatin has radiosensitivity; its mechanism may be related to the promotion of hepatocellular carcinoma cell apoptosis and cell G2 / M arrest.