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目的 研究负载肿瘤抗原的树突状细胞活化的特异性细胞毒性T淋巴细胞对原发性肝癌细胞的杀伤效应 ,探讨其用于临床治疗的可行性 ,并为其应用提供实验依据。方法 联合应用粒细胞 /巨噬细胞集落刺激因子、白细胞介素 - 4和肿瘤坏死因子 -α等 ,对大鼠骨髓单个核细胞进行体外诱导 ,同时加入大鼠原发性肝癌细胞株CBRH- 7919细胞匀浆粗提物进行刺激 ,制备负载肿瘤抗原的树突状细胞后 ,活化同基因大鼠的脾脏细胞 ,制备特异性细胞毒性T淋巴细胞 ,并采用MTT法检测效应细胞对CBRH - 7919的杀伤效果 ,同时与效应细胞对K5 6 2的杀伤率进行比较。结果 大鼠骨髓单个核细胞诱导的负载肿瘤抗原的树突状细胞活化的特异性细胞毒性T淋巴细胞对CBRH - 7919具有较高的特异性杀伤作用。结论 负载肿瘤抗原的树突状细胞对原发性肝癌的治疗具有潜在的临床应用前景。
Objective To study the killing effect of specific cytotoxic T lymphocytes activated by dendritic cells loaded with tumor antigens on primary hepatocellular carcinoma cells, to explore the feasibility of their use in clinical treatment, and to provide experimental basis for their application. Methods Combined use of granulocyte/macrophage colony stimulating factor, interleukin-4 and tumor necrosis factor-alpha, etc., the rat bone marrow mononuclear cells were induced in vitro, and rat primary hepatoma cell line CBRH-7919 was added simultaneously. The crude extracts of cell homogenate were stimulated to prepare dendritic cells loaded with tumor antigens, and then the spleen cells of syngeneic rats were activated to prepare specific cytotoxic T lymphocytes, and effector cells were detected by MTT assay on CBRH-7919 cells. The killing effect was compared with the K562 killing rate of the effector cells. Results Specific cytotoxic T lymphocytes activated by dendritic cells loaded with tumor antigens induced by rat bone marrow mononuclear cells had a high specific killing effect on CBRH-7919. Conclusion The dendritic cells loaded with tumor antigens have potential clinical applications for the treatment of primary liver cancer.