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目的:研究褪黑素对病毒唑体外抗呼吸道合胞病毒(RSV)的协同作用。方法:采用MTT比色法,观察2种药物联用时对RSV在人喉癌上皮细胞(Hep-2)所致细胞病变的抑制效应,统计学及金正均法q值判断2种药物的相互作用。结果:病毒唑对RSV具有抑制作用,且呈浓度依赖的趋势;褪黑素几乎没有抗RSV活性,但能增强病毒唑对RSV的抑制作用;2种药物合用对病毒的抑制作用明显增强,褪黑素(25、50mg·L-1)分别与病毒唑(50、100、200mg·L-1)合用时,q值均>1.15,呈现协同作用。结论:病毒唑与褪黑素联合可协同增强对RSV的抑制作用。
Objective: To study the synergistic effect of melatonin on ribavirin against respiratory syncytial virus (RSV) in vitro. Methods: MTT colorimetric method was used to observe the inhibitory effect of RSV on cytopathic effect induced by human laryngeal squamous cell carcinoma (Hep-2) when the two drugs were used in combination. Statistical analysis and q value of Kim’s method were used to determine the interaction between the two drugs . Results: Ribavirin had an inhibitory effect on RSV in a concentration-dependent manner. Melatonin had almost no anti-RSV activity, but enhanced inhibitory effect of ribavirin on RSV. The inhibitory effect of ribavirin on the virus was significantly enhanced and faded When combined with ribavirin (50, 100, 200 mg · L-1), the q values were both> 1.15 for melanin (25 and 50 mg · L-1), respectively, showing a synergistic effect. Conclusion: Combination of ribavirin and melatonin can synergistically enhance the inhibitory effect on RSV.