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目的考察痛觉敏感性与体内雌孕激素水平的可能关系。方法将大鼠分为去卵巢组(8只)和去卵巢后雌激素替代组(8只),去卵巢组通过去卵巢手术降低大鼠体内卵巢激素水平,去卵巢后雌激素替代组在去卵巢手术后1周给予外源性雌激素替代。分别在两组大鼠足底注射P2Y1选择性激动剂2-MeSADP,通过行为学实验观察对大鼠足底机械痛阈的影响,并通过实时定量PCR研究2组大鼠背根神经节(DRG)中P2Y1受体mRNA表达的差别。结果与去卵巢组大鼠相比,雌激素替代组大鼠机械痛阈增高(P=0.014);足底注射P2Y1选择性激动剂2-MeSADP后,去卵巢组大鼠在注射药物前后痛阈无统计学差异,而雌激素替代组大鼠出现痛觉增敏。实时定量PCR结果表明雌激素替代组大鼠DRG P2Y1受体mRNA的表达高于去卵巢组大鼠(P<0.05)。结论雌激素可能通过促进DRG上P2Y1受体的表达从而影响外周机械痛阈。
Objective To investigate the possible relationship between hyperalgesia and estrogen and progesterone in vivo. Methods The rats were divided into ovariectomized group (n = 8) and ovariectomized estrogen replacement group (n = 8). The ovariectomized group was ovariectomized to reduce the level of ovarian hormone in the ovariectomized rats. The estrogen replacement group One week after ovarian surgery, exogenous estrogen replacement was given. The P2Y1 selective agonist 2-MeSADP was injected into the soles of both groups respectively, and the mechanical threshold of the plantar threshold was observed by behavioral experiments. The DRG ) P2Y1 receptor mRNA expression differences. Results Compared with ovariectomized rats, the mechanical pain threshold was increased in the estrogen replacement group (P = 0.014). After the P2Y1 selective agonist 2-MeSADP was injected into the plantar, the pain threshold of the ovariectomized rats No statistical difference, while estrogen replacement group rats sensitized hyperalgesia. Real-time quantitative PCR results showed that the expression of DRG P2Y1 receptor mRNA in estrogen replacement group was higher than that in ovariectomized group (P <0.05). Conclusion Estrogen may influence peripheral mechanical pain threshold by promoting the expression of P2Y1 receptor on DRG.