目的探讨实验性肝纤维化发生发展过程中肝脏纤维结合蛋白与糖胺金糖、胶原积聚的相互关系．方法取大鼠８０只，应用复合致病因子复致大鼠实验性肝硬变模型，于实验第２、４、６、８Ｗ末处死动物，测定肝脏胶原蛋白及糖胺多糖含量，应用免疫组化及图像分析法观察肝脏纤维结合蛋白的变化．正常大鼠为对照．结果肝纤维化组大鼠第２，４，６，８Ｗ末肝脏纤维结合蛋白、糖胺多糖及胶原含量均较正常大鼠显著升高（Ｐ＜００１），同时肝纤维化形成期（实验第４Ｗ末）肝内纤维结合蛋白分布面积与糖胺多糖及胶原含量均呈显著正相关（ｒ胶原＝０８９９，ｒ糖胺多糖＝０９１３，Ｐ＜００５）．结论肝纤维化形成过程中肝内纤维结合蛋合、糖胺多糖及胶原的沉积存在着量的相关性，三者可能通过协同作用共参与肝纤维间隔的形成 Objective To investigate the relationship between hepatic fibronectin and glycosaminoglycan and collagen accumulation during the development of experimental hepatic fibrosis. Methods Totally 80 rats were used and the compound pathogenic factor was used to induce experimental model of liver cirrhosis in rats. Animals were killed at the end of the 2nd, 4th, 6th and 8th weeks. The contents of collagen and glycosaminoglycan in the liver were measured. Changes of liver fibronectin were observed by image analysis. Normal rats as controls. Results The content of fibronectin, glycosaminoglycan and collagen in the 2nd, 4th, 6th and 8th liver of rats with liver fibrosis was significantly higher than that of normal rats (P <001), and the formation of liver fibrosis At the end of experiment 4W, the distribution area of ​​fibronectin in liver was significantly and positively correlated with glycosaminoglycan and collagen content (r collagen = 0.899, r glycosaminoglycan = 0.913, P <005). Conclusions There is a correlation between the deposition of intrahepatic fibronectin, glycosaminoglycans and collagen during the formation of hepatic fibrosis. The three may participate in the formation of hepatic fibril septa through synergistic effect