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目的:筛选乌头子根组织培养芽萌发的最佳抗褐化剂。方法:利用乌头子根的芽眼为外植体,在组培芽的萌发阶段,考察不同浓度配比的聚乙烯吡咯烷酮(PVP)、活性炭(AC)、硝酸银(AgNO_3)和硫代硫酸钠(Na_2S_2O_3)对乌头子根芽培养中抗褐化的影响。结果:褐化集中发生在接种的10 d内,AC能有效控制褐化,乌头子根外植体在4 g/L AC培养基中5 d时的褐化率最低(7.7%),10 d时褐化率仅为22.1%,此浓度下外植体萌发率也最高(81.7%)。4种抗褐化剂对外植体生长影响方面,AgNO_3处理的芽眼生长状态较好,浓度4 mg/L处理的芽眼可分化丛生芽3个,分化率为24.2%。结论:乌头子根芽眼的组织培养中,先在添加4 g/L AC的培养基中培养10 d后,转入添加4 mg/L AgNO_3的培养基,在有效控制褐化的同时,促进外植体的生长与增殖。
OBJECTIVE: To screen the best browning agent for buds germinated from root tissue culture of Aconitum kusnezoffii. Methods: Using the bud of the root of Aconitum kusnezoffii as explant, the effects of different concentrations of polyvinyl pyrrolidone (PVP), activated carbon (AC), silver nitrate (AgNO 3) and sodium thiosulphate Effect of Na_2S_2O_3 on Antibrowning in the Root Bud Culture of. Results: Browning took place within 10 days after inoculation. AC could effectively control browning. The browning rate of AChE explants was lowest (7.7%) in 4 g / L AC medium for 5 d, When the browning rate was only 22.1%, the highest rate of explant germination at this concentration (81.7%). In the aspect of the effect of four kinds of browning agents on the growth of explants, AgNO_3 treated buds grew well, and 3 buds with 4 mg / L concentration could differentiate into buds with a differentiation rate of 24.2%. CONCLUSION: The tissue culture of the root of the aconite root was first cultured for 10 days in the medium supplemented with 4 g / L AC and then transferred to the medium supplemented with 4 mg / L AgNO3 to effectively control the browning while promoting Implant growth and proliferation.