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化学合成Ｎ端含多个赖氨酸残基、Ｃ端为人碱性成纤维生长因子（ｂＦＧＦ）的受体结合的２６肽（ＦＤＫｇ）；经细 胞培养、基因转染、报道基因检测等实验。结果表明，合成肽ＦＤＫ９能通过电性中和与ＤＮＡ分子结合，ＤＮＡ凝胶阻 滞试验表明浓度为Ａ＝１的ＦＤＫ９肽每４μＬ能与１μＬ萤火虫荧光素酶表达质粒ｐＥＢｌｕｃ完全结合，核酸复合物直接加入 人脐静脉内皮细胞的培养液中， ４８ｈ后可从细胞裂解液中测出荧光素酶的显著表达，而缺乏 ｂＦＧＦ受体的 Ａ４３１细胞 裂解液无荧光素酶的表达。表明已成功建立了ｂＦＧＦ受体介导的基因转移系统，推测该系统可特异地将外源基因导入 ＦＧＦ受体过量表达的再狭窄动脉壁。
Chemically synthesize 26-mer peptide (FDKg) with multiple lysine residues at the N-terminus and receptor binding of the human basic fibroblast growth factor (bFGF) at the C-terminus. Cell culture, gene transfection and reporter gene assay were also performed. The results showed that the synthetic peptide FDK9 could be electrically neutralized with the DNA molecule. The DNA gel retardation test showed that the FDK9 peptide with the concentration of A = 1 could be bound with 1μL of firefly luciferase expression plasmid pEBluc every 4μL. The nucleic acid complex Directly added to the culture medium of human umbilical vein endothelial cells, significant expression of luciferase was detected in cell lysates 48h later, while the lack of bFGF receptor A431 cell lysates without luciferase expression. This indicates that the bFGF receptor-mediated gene transfer system has been successfully established and it is speculated that this system can specifically introduce foreign genes into the restenosis artery wall where FGF receptor is overexpressed.