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目的建立同时测定鸡骨香Croton crassifolius中6个萜类成分(chettaphanin I、山藿香定、crassifolin B、cyperenoic acid、crassifolin A、cyperenol)的HPLC方法。方法采用Kromasil 100-5 C18色谱柱(250 mm×4.6 mm,5μm);以乙腈(A)-0.02%三氟乙酸水(B)梯度洗脱:0~35 min,35%A;35~55 min,35%~60%A;55~80 min,60%A。体积流量为1.0 m L/min;检测波长为210 nm;柱温为25℃。结果 6个萜类化合物均有良好的分离度,线性关系良好(r>0.999 7),chettaphanin I、山藿香定、crassifolin B、cyperenoic acid、crassifolin A、cyperenol的加样回收率分别为100.2%、99.13%、98.48%、99.22%、101.1%、102.5%,RSD分别为0.48%、0.48%、0.96%、1.10%、1.35%、0.95%。结论该方法简单准确,具有良好的重复性和稳定性,可为鸡骨香质量控制提供科学依据。
Objective To establish an HPLC method for simultaneous determination of six terpenoids (chettaphanin I, crassifolin B, cyperenoic acid, crassifolin A, cyperenol) in Croton crassifolius. Methods The elution was performed on a Kromasil 100-5 C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution from acetonitrile (A) to 0.02% trifluoroacetic acid (B): 0 to 35 min, 35% A, 35 to 55 min, 35% -60% A; 55-80 min, 60% A. The volume flow rate was 1.0 m L / min. The detection wavelength was 210 nm. The column temperature was 25 ℃. Results All the terpenoids showed good linearity (r> 0.999 7). The recoveries of chettaphanin I, patchouline, crassifolin B, cyperenoic acid, crassifolin A and cyperenol were 100.2% , 99.13%, 98.48%, 99.22%, 101.1% and 102.5% respectively. The RSDs were 0.48%, 0.48%, 0.96%, 1.10%, 1.35% and 0.95%, respectively. Conclusion The method is simple and accurate, with good repeatability and stability, which can provide a scientific basis for the quality control of chicken bone.