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【目的】探讨在戊四氮(pentetrazole,PTZ)致癫痫大鼠海马组织中Homer1a、mGluR5的表达以及丙戊酸钠(sodium valproate,VPA)对其表达的影响。【方法】将SD大鼠分为正常对照组(NC)、PTZ组和VPA干预组。PTZ腹腔注射达到点燃标准,腹腔注入VPA 15mg/(kg·d),对致痫大鼠进行治疗;30min后,腹腔注射PTZ诱发癫痫发作;两周后分别于致痫大鼠发作后8h、24h处死大鼠;应用实时定量PCR(realtime-PCR)、Western blot技术检测海马组织Homer1a、mGluR5的表达。【结果】1)NC组无癫痫发作,VPA干预组癫痫发作较PTZ组减轻;2)PTZ组Hmoer1a于癫痫发作后8h明显升高,24h后则明显降低;VPA干预组Homer1a表达于癫痫发作后8h及24h均升高;3)PTZ组mGluR5表达于癫痫发作后8h无显著变化,而24h后明显升高;VPA干预组mGluR5表达于癫痫发作后8h无显著变化,24h后明显降低。【结论】1)丙戊酸钠可明显改善致痫大鼠发作表现;2)Homer1a在癫痫发作后早期短暂性升高;mGluR5在癫痫发作后早期无明显变化,而发作后24h升高;3)丙戊酸钠可能通过调节Homer1a表达进一步影响mGluR5而发挥抗痫作用。
【Objective】 To investigate the expression of Homer1a and mGluR5 and the effect of sodium valproate (VPA) on the expression of hippocampal neurons in pentetrazole (PTZ) -induced epileptic rats. 【Methods】 SD rats were divided into normal control group (NC), PTZ group and VPA intervention group. PTZ intraperitoneal injection to achieve the ignition standard, intraperitoneal injection of VPA 15mg / (kg · d), epileptic rats for treatment; 30min after intraperitoneal injection of PTZ induced seizures; two weeks after the onset of seizures in rats 8h, 24h The rats were sacrificed. The expression of Homer1a and mGluR5 in hippocampus was detected by realtime-PCR and Western blot. [Results] 1) NCI group had no seizures and VPA intervention group had less seizure than PTZ group; 2) Hmoer1a in PTZ group increased significantly at 8h after seizure and decreased significantly after 24h; In VPA intervention group, the expression of Homer1a increased after seizure 8h and 24h respectively. 3) The expression of mGluR5 in PTZ group did not change significantly at 8h after seizure, but increased significantly after 24h. The expression of mGluR5 in VPA intervention group had no significant change at 8h after seizure onset and decreased significantly after 24h. 【Conclusion】 1) Sodium valproate can significantly improve the seizure performance of epileptic rats; 2) Homer1a transiently increases in the early post-seizure phase; mGluR5 has no obvious change in the early post-seizure phase and increases 24h post-seizure; 3 ) Sodium valproate may play an anti-epileptic effect by regulating Homer1a expression to further affect mGluR5.