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目的建立转铁蛋白修饰的阿霉素脂质体在小鼠体内组织浓度的测定方法。方法选择乙腈-5mmol·L-1乙酸铵水溶液(30:70)为流动相;色谱柱:DIKMA Diamonsil C18柱(4.6mm×150mm,5μm);流速1.0mL·min-1;检测波长:激发波长为480nm,发射波长为550nm;柱温:30℃。结果本实验所用方法线性关系良好,且具有专一性强、准确性、灵敏度高的优点,符合生物样本分析的要求。结论可用本法快速、简便、准确地测定小鼠组织样品中转铁蛋白修饰的阿霉素脂质体的含量。
Objective To establish a method for the determination of the tissue concentration of transferrin-modified doxorubicin liposome in mice. Methods The mobile phase was acetonitrile-5mmol·L-1 ammonium acetate solution (30:70). The column was DIKMA Diamonsil C18 (4.6mm × 150mm, 5μm), the flow rate was 1.0mL · min-1. 480nm, emission wavelength of 550nm; column temperature: 30 ℃. Results The method used in this experiment has a good linear relationship, and has the advantages of specificity, accuracy and sensitivity, and meets the requirements of biological sample analysis. Conclusion This method can be used to determine the content of transferrin-modified doxorubicin liposomes in mouse tissue samples quickly, easily and accurately.