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以玉米为材料，以植物质膜水孔蛋白的抗血清为探针，运用免疫荧光技术，在激光共聚焦显微镜下观察了水孔蛋白在玉米根原生质体细胞膜上的分布情况．结果表明，玉米根原生质体细胞膜富含水孔蛋白，它们在细胞膜上的分布具有不均一性． Ｗｅｓｔｅｒｎ ｂｌｏｔｔｉｎｇ实验进一步表明，玉米根质膜的水孔蛋白主要以二聚体或二聚体与单体的混合形式存在，分子量分别为５５和２８ ｋｕ．原生质体膨胀实验表明，水孔蛋白的抑制剂ＨｇＣＩ２及植物质膜水孔蛋白的抗血清可以明显抑制原生质体在低渗溶液中的膨胀，进一步说明了玉米根质膜水孔蛋白是一种汞敏感的水孔蛋白，在水分跨玉米原生质体细胞膜的运输中着重要作用．
Using maize as material and antisera of plant plasma membrane aquaporin as probe, the distribution of aquaporin on the membrane of maize protoplast was observed under laser scanning confocal microscope with immunofluorescence technique. The results showed that the plasma membrane of maize root protoplasts is rich in aquaporins, and their distribution on the cell membrane is heterogeneous. Western blotting experiments further showed that the aquaporin of maize root plasma membrane mainly exists as a dimer or a mixture of dimers and monomers with molecular weights of 55 and 28 ku, respectively. Protoplast swelling experiments showed that the water pore protein inhibitor HgCI2 and plant plasma membrane aquaporin antisera can significantly inhibit the expansion of protoplasts in hypotonic solution, further illustrates that the corn rhizosphere aquaporin is a mercury Sensitive aquaporins play an important role in the transport of moisture across the cell membrane of maize protoplasts.