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目的:探讨中药红藤方对异位子宫内膜的抑制作用。方法:用手术自体移植法成功建立的子宫内膜异位症大鼠模型,随机分成5组:红藤方高剂量组(80g生药/kg,A组)、低剂量组(20g生药/kg,B组)、达那唑组(60mg/kg,C组)、去势组(D组)和模型组(E组),连续给药21d后,检测各组的血清E2水平及异位内膜和卵巢的芳香化酶的变化;另测定异位内膜的体积。结果:A组、C组和D组的异位内膜生长均明显受到抑制,且3组间无统计学差异(P>0.05),与E组比有统计学差异(P<0.05);组织学观察可见,异位内膜呈退化趋势,A、C和D组间大鼠血清中的E2水平无统计学差异(P>0.05),与E组相比有统计学差异(P<0.05);A组、C组及D组异位内膜、卵巢组织的芳香化酶P450低表达,A组与E组间有统计学差异(P<0.05)。结论:红藤方可通过抑制异位内膜、卵巢的芳香化酶活性表达,降低局部雌激素含量,抑制异位内膜的生长,使异位内膜萎缩。
Objective: To investigate the inhibition of ectopic endometrium by Chinese medicine Hongteng Fang. METHODS: The rat model of endometriosis that was successfully established by autologous transplantation was randomly divided into five groups: high-dose group of Hongtengfang (80g crude drug/kg, group A) and low-dose group (20g crude drug/kg, Group B), danazol group (60 mg/kg, group C), castration group (group D), and model group (group E), serum E2 levels and ectopic endometrium were measured after 21 days of continuous administration. Changes in the aromatase of the ovaries; the volume of the ectopic endometrium was also determined. Results: The ectopic endometrial growth in group A, C and D was significantly inhibited, and there was no statistical difference between the three groups (P>0.05). There was a statistically significant difference between the three groups (P<0.05). Observations showed that the ectopic endometrium showed a degenerative trend. There was no significant difference in serum E2 levels between groups A, C and D (P>0.05). There was a statistically significant difference compared with E group (P<0.05). ; A group, C group and D group ectopic endometrium, ovarian tissue aromatase P450 low expression, A group and E group were statistically significant difference (P <0.05). Conclusion: Hong Tengfang can inhibit the growth of ectopic endometrium by inhibiting the expression of aromatase activity in ectopic endometrium and ovaries, reducing the local estrogen content, and atrophy of the ectopic endometrium.