本文介绍了体内测定外用抗菌剂作用的定量试验方法,用这些方法能在机体全面地估价受检制剂的抗菌活性,以用于浅表感染的治疗、手消毒和预防伤口及擦伤感染等。受试者先做封包试验(Occlusion test),以了解抗菌剂是否具有抗菌活性及足够的稳定性来阻止寄居菌群的繁殖。封包试验的原理是前臂的菌群是稀少的(每平方厘米为10~1～10~2),用不通透性封包后能增加皮肤表面湿度,使细菌繁殖,通常经48小时后菌量可达10~6/平方厘米。其方法是用注射器将0.1毫升受检物分别滴于每只前臂的2个方块(5×5厘米)上,取0.1毫升赋形剂置每侧前臂的第 This paper presents a quantitative test method for the in vivo determination of topical antibacterial agents. These methods provide a comprehensive assessment of the antibacterial activity of the tested agents in the body for the treatment of superficial infections, hand disinfection and prevention of wounds and abrasions. Subjects did an Occlusion test to see whether the antibacterial agent had antibacterial activity and sufficient stability to prevent the colonization of resident colonies. The principle of the package test is the forearm flora is sparse (per square centimeter for the 10 ~ 1 ~ 10 ~ 2), with non-permeability package can increase the skin surface humidity, the bacteria multiply, usually after 48 hours of bacteria Up to 10 ~ 6 / square centimeter. The method is to inject 0.1 ml of the test substance into two blocks (5 × 5 cm) of each forearm with a syringe respectively, take 0.1 ml of the vehicle